Cell and Tissue Journal

Abstract Aim: This research, design and fabrication of a composite scaffold for growth, proliferation and differentiation of Cardiac Progenitor Cells (CPCs) has been considered.
Material and method: Polycaprolactone / Gelatin composite scaffolds with a ratio of 70:30 and with the most similarities to the cardiac extracellular matrix was fabricated with aligned nanofibers. Using scanning electron microscopy (SEM), mechanical strength analysisand also contact angel test, the scaffold was investigated due to have the most necessary characteristics to be proportional for cardiac scaffold. Finally, By Real time-PCR test, the expression of the specific genes related to the Cardiac Progenitor Cells differentiation (MYH-6, TTN and CX-43) has been analyzed.
Results: Based on our results from contact angle test and mechanical strength experiments, we concluded that our designed scaffold is suitable for the culture of Cardiac Progenitor Cells on it. The Real time-PCR analysis also showed the good expression of genes associated with contraction.
Conclusion: What makes the cardiomyocytes different from other cells is their contraction related to specific cardiac genes being responsible for beating synchronization. The results of this study showed that aligned Polycaprolactone / Gelatin composite scaffold has the appropriate potential for induction of cardiac progenitor cells differentiation and application in heart tissue engineering.
 

Abstract Aim: The purpose of the current study is to investigate the cytotoxic and oxidative effects of Salvia officinalis hydroalcoholic extract on cancer A549 cells.
Material and Methods: The cells were seeded in plates to investigate concentrations of Salvia officinalis extract and MTT measurement was done to determine IC50 concentration and cell viability on days 1, 3, 5, and 7. Moreover, analyses of superoxide dismutase and catalase enzymes involved in oxidative stress pathway, and AO/EB staining for a qualitative investigation of cell lines has been conducted in order to explore the effects of IC50 concentration on apoptosis induction. Also, Giemsa and DAPI stainings were utilized to explore the morphological changes of cell and nucleus..
Results: IC50 concentration of Salvia extract for A549 cells was determined 5 mg/mL. According to the results, Salvia extract reduced the cell viability of A549 cells. Based on the results, Salvia extract had a significantly greater cytotoxic effect compared to the control sample of A549 cell line. Treatment cells indicated some clear and dose-dependent differences at different times. The results of stainings proved the apoptosis induction in the treatment group. Furthermore, regarding the enzyme expression results the activity of superoxide dismutase and catalase enzymes in the cell groups treated by Salvia extract was significantly increased, compared to the control group.
Conclusion: Consistent with the results of this study, in addition to the anti-oxidative activity Salvia officinalis hydroalcoholic extract revealed significant apoptotic effects on lung cancer cells, considering a time and dose-dependent method.
 

Abstract Aim: The aim of this experimental study was to investigate the effective infertility factors of physoaline compounds on the adult reproductive system of Balb/C adult female.
Material and Methods: The above experiment was performed by preparing the aqueous extract of the plant and selecting three doses of 7.5g/kg,9g/kg and 15g/kg;Simultaneously with the experimental groups,the distilled water sham group was injected and the control group was kept intact.Vaginal stromal smear cycles were prepared to examine different stages.One hour after the last injection,the mice were given blood samples and then killed by chloroform and their uterus and ovaries were prepared for histological studies.
Results: Statistical studies have shown that injecting 7.5g/kg of extract reduces estrogen and restores 33% of fertility in mice. Also, at a dose of 9 g/kg, the mice were initially infertile, but one month after the last injection, 16.5% of the mice showed a return to fertility and a significant increase in progesterone.
Conclusion: According to the results, it is probably because physalines have a pseudo-steroidal structure. The receptor is occupied by physaline and can cause abnormalities in the growth and development of oocytes, uterine tissue cells and ovaries, and infertility.
 

Abstract Aim: The current study aims to utilize varicocele induction in male rat models to evaluate effect and impact of varicocele on DNA integrity.
 
Material and Methods: 66 male Wistar rats were divided into 3 groups: control, sham and varicocele-induced (VI). 2 months post-VI, Sperm parameters, percentage of sperm DNA damage, persistent histone and lipid peroxidation were assessed.
 
Results: Animal weight and their epididymal length shown no significant changes but there were significant differences in testis volume and sperm parameters between VI group versus control and sham groups. Similarly, increased level of DNA damage, persistent histone and lipid peroxidation were evaluated, and compared between varicocele and control group.
 
Conclusion: Based on literature and the result of this study, it is likely that increase oxidative stress following varicocele induction leads to lipide peroxidation, DNA damages and reduced sperm parameters. In the current study, by taking the advantage of homogenous genetic background and high number of animals, we showed that varicocele have damaging effect on DNA and chromatin integrity and should be reversed in human before aiming for pregnancy.
 
 

Abstract The electrical functions of the organs of the body, such as the nervous system and the bone marrow, has led to the use of one of the most widely used therapies called electrotherapy, especially in relieving pain. On the other hand, advancement of medical science in the field of stem cells and regenerative medicine has made many perspective in treatment. Electric field-based therapies have recently been widely used in the treatment of cancer. The main issues in regenerative medicine are the proliferation of stem cells to the required extent and their guidance towards differentiation into the target tissue. Electric field stimulation (EF) can also play an important role in generating appropriate stem cell responses and guiding stem cell differentiation through osteogenesis/neurogenesis/cardiomyogenesis. Nanosecond pulsed electric field as well as the tumor treating field have attracted a lot of attention today for the treatment of cancer. Major signaling pathways and cellular responses elicited by electrical stimulation are included reactive oxygen species and heat shock proteins, intracellular calcium ion fluctuation, so ATP production, clustering or re-accumulation of cell surface receptors, Skeletal regeneration and so on that they can affect the stem cell fate. Also, none invasive, ease of usage, and reasonable price have caused that the treatment of cancer with an electric field to be increasingly used. This study seeks to provide a brief overview of the effects of electrical signals on the behavior of stem cells, as well as examples of their therapeutic effects in the treatment of tissue lesions and cancer.
 

Abstract Aim: In current study was aimed to investigate effects of different concentrations Genestein on osteogenic differentiation and proliferation of adipose driven mesenchymal stem cells (ADMSCs) from Turkman horses.
Material and Methods: In this experimental study, ADMSCs were treated with 10^-8,10^-7,10^-6,10^-5 μM concentrations of Genestein with osteogenic medium. The proliferation and cell viability of Genestein was evaluated by MTT test for 7 days after cell culture, and the role of It on osteogenic differentiation was investigated by alizarin red staining, alkaline phosphatase assay and calcium content for 21 days. The data were analyzed with One-way ANOVA.
Results: The results of MTT test showed after treatment of cells by Genestein, it had no toxic effect (p < 0.05). The activity of alkaline phosphatase in 10-5 concentration treated   cells was higher than the other which attributed to the end of differentiation process (21th day) (p < 0.05). The results of alizarin red staining and calcium content showed that the maximum level of Genestein has significantly increase of precipitation of mineral ions on extracellular matrix on 14th and 21st days compare other concertation.
Conclusion: According to the results of this study, Genestein promoted differentiation of these cells in to osteoblast cells without any toxicity effect.