Research - Scientific
Investigation of Cell Cycle, Myogenic and Growth Factor Genes Expression in Ovine Primary Myoblast Cells by Follistatin Overexpression

M Nazari; F Salabi; L Du

Volume 8, Issue 1 , July 2017, Pages 1-11

https://doi.org/10.52547/JCT.8.1.1

Abstract
  Aim: The aim of this study was investigation of Follistatin gene over-expression effects on cell cycle, myogenic and growth factor genes expression of ovine primary myoblast (OPM) cells in lab that was done using AAV serotype 2 virus (rAAV2) carrying Follistatin (FST). Material and Methods: Obtained ...  Read More

Research - Scientific
Evaluation of myelin sheath and their related proteins (MAG, MOG, MBP and PLP) during cuprizone-induced demyelination in central nervous system of male C57BL/6 mice

l n Sanadgo; i M komijan

Volume 8, Issue 1 , July 2017, Pages 12-21

https://doi.org/10.52547/JCT.8.1.12

Abstract
  Aim: The aim of this study was to investigate of cuprizone time-dependent effects on MAG, MOG, MBP, and PLP proteins and demyelination process. Materials and Methods: The myelin degradation model was created with a five week diet containing 0.2% of cuprizone. The rats brain corpus callosum region was ...  Read More

Research - Scientific
Investigation of the Effect of Inhalation of Magnetic Iron Oxide Nanoparticles on the Expression of P53 in the Testis Tissue of Balb/C Mice by Immunohistochemically Staining

Z Nasiri; S.J Moshtaghian; F Esmaeili

Volume 8, Issue 1 , July 2017, Pages 22-31

https://doi.org/10.52547/JCT.8.1.22

Abstract
  Aim: The present study was designed to investigate the effects of chitosan/poly vinyl alcohol scaffold incorporated by NRBE (neonatal rat brain extract) on neuronal differentiation of P19 EC (embryonal carcinoma) stem cells. Material and Methods: In order to induce neuronal phenotype, P19-derived embryonic ...  Read More

Research - Scientific
Study the effects of some acute environmental conditions on recombinant Hsp70 protein expression in Rutilus Frissi Kutum liver in E. coli

O Saberi; H Ghafoor

Volume 8, Issue 1 , July 2017, Pages 32-41

https://doi.org/10.52547/JCT.8.1.32

Abstract
  Aim: The aim of the present study was to investigate the optimal expression of Hsp70 recombinant Rutilus FrissiKutum liver in E. coli. Material and methods: To evaluating of expression level of recombinant Hsp70, transformed E. coli were cultured in LB medium at 2, 4, 8, 12, 24, 36 and 48 hours, 20, ...  Read More

Research - Scientific
Effect of Glycyrriza glabra L. extract on ODC1 gene expression and cell proliferation in human breast cancer cells

A Safipour Afshar; F Saeid Nematpour; M Lakzian

Volume 8, Issue 1 , July 2017, Pages 42-51

https://doi.org/10.52547/JCT.8.1.42

Abstract
  Aim: Product of ODC1 gene is key enzyme in polyamines synthesis that in diverse cancers their content increases. In the present study the effects of root extract of licorice on the ODC1gene expression and cell viability of two human breast cancer cell lines (MCF-7 and MDA-MB-231) and non-cancer cell ...  Read More

Research - Scientific
Influence of Occupational Exposures on Sperm Parameters and Chromatin Structure

L Babakhah; i L Azad; M Arbabian; M Tavalaee; M Bahadorani; MH Nasr- Esfahani

Volume 8, Issue 1 , July 2017, Pages 52-68

https://doi.org/10.52547/JCT.8.1.52

Abstract
  Aim: This study examines the sperm parameters (concentration, mobility and morphology) and the state of sperm DNA in infertile men exposed to various occupational exposures. Material and Methods: This study was performed on 152 men who referred to Isfahan Fertility and Infertility Center )spring 2014 ...  Read More

Research - Scientific
Antibacterial effect of Modified Zeolite with Schiff base

M Zendehdel; Z Mortezaei; M Yusefi

Volume 8, Issue 1 , July 2017, Pages 69-76

https://doi.org/10.52547/JCT.8.1.69

Abstract
  - Aim: In this study, solid hybrid material from the Schiff base complex was prepared inside and on the surface of NaY zeolite, and then their antibacterial properties against different bacteria (gram-positive and gram negative bacteria) was investigated. Material and Methods: Antibacterial compounds ...  Read More

Research - Scientific
Purification of Influenza A (H1N1) Recombinant Nucleoprotein , Preparation and Purification of its Polyclonal Antibody in Rabbits

i M Sharaf; Z Asgariazadeh; MA Karimi Torshizi

Volume 8, Issue 1 , July 2017, Pages 77-87

https://doi.org/10.52547/JCT.8.1.77

Abstract
  - Aim: This study was carried out to investigate the application of different magnetic intensities on the sperm freezing medium. Material and methods: In this experiment, the magnetic field induction of 0, 2000, 4000 and 6000 Gauss was used to determine the optimum intensity during the freeze-thawing ...  Read More

Research - Scientific
Investigating of protective effects of Nurr1 and GDNF on dopaminergic neural cell line SH-SY5Y against neuro-inflammation and toxicity of 6-OHDA

M Rasoolnezhad; M Gardaneh; Sabouni F

Volume 8, Issue 1 , July 2017, Pages 87-108

https://doi.org/10.52547/JCT.8.1.87

Abstract
  - Aim: In this study Nurr1 and GDNF, due to their anti-inflammatory and regenerative effects and Nurr1-mediated regulation of GDNF receptor (Ret) expression, were selected to protect dopaminergic SH-SY5Y cell line against neuroinflammation and toxicity caused by 6-OHDA. Material and Methods: Recombinant ...  Read More