Arak University Cell and Tissue Journal 2228-7035 2 4 2012 03 01 Evaluation of the Phytoremediation of Robinia pseudoacacia L. in Petroleum- contaminated Soils with Emphasis on the Some Heavy Metals Evaluation of the Phytoremediation of Robinia pseudoacacia L. in Petroleum- contaminated Soils with Emphasis on the Some Heavy Metals 437 442 681 10.52547/JCT.2.4.437 FA Journal Article 2012 09 11 Aim: Petroleum is a complex mixture of thousands of hydrocarbon and non-hydrocarbon compounds, including heavy metals which potentially are carcinogenic and mutagenic. Phytoremediation has been shown to be effective for degradation or removing petroleum contaminants. Selection of plant species for phytoremediation, however, is complicated. <br />Material and Methods: The effects of petroleum pollution of soil (0%, 1%, 2%, 3% and 4% V/W) on the proline, total protein , lead, cadmium and zinc contents  in Acacia leaveswere investigated using methods of Bathes, Bradford and Atomic Absorption, respectively. The data were statistically analyzed using SPSS v11 and Duncan’s test. <br />Results: The results showed that the total protein and proline contents increased significantly (p≤0.05) as the level of pollution increased. The highest proline content was measured at 4% treated plants. Proline accumulation is a common physiological response in many plants against a wide range of biotic and abiotic stresses. The results showed that lead and zinc accumulated by leaves. The leaf lead values were markedly, 20.8-fold, enhanced in 1%. There was not significant differences among cadmium levels in treatments and the control plants. <br />Conclusion: Based upon these results, <em>Robinia pseudoacacia</em> L. can be used as bioaccumulator in petroleum pollution and were selected for the further investigation of the phytoremediation of pb-contaminated soil. <br />  Aim: Petroleum is a complex mixture of thousands of hydrocarbon and non-hydrocarbon compounds, including heavy metals which potentially are carcinogenic and mutagenic. Phytoremediation has been shown to be effective for degradation or removing petroleum contaminants. Selection of plant species for phytoremediation, however, is complicated. <br />Material and Methods: The effects of petroleum pollution of soil (0%, 1%, 2%, 3% and 4% V/W) on the proline, total protein , lead, cadmium and zinc contents  in Acacia leaveswere investigated using methods of Bathes, Bradford and Atomic Absorption, respectively. The data were statistically analyzed using SPSS v11 and Duncan’s test. <br />Results: The results showed that the total protein and proline contents increased significantly (p≤0.05) as the level of pollution increased. The highest proline content was measured at 4% treated plants. Proline accumulation is a common physiological response in many plants against a wide range of biotic and abiotic stresses. The results showed that lead and zinc accumulated by leaves. The leaf lead values were markedly, 20.8-fold, enhanced in 1%. There was not significant differences among cadmium levels in treatments and the control plants. <br />Conclusion: Based upon these results, <em>Robinia pseudoacacia</em> L. can be used as bioaccumulator in petroleum pollution and were selected for the further investigation of the phytoremediation of pb-contaminated soil. <br />  https://jct.araku.ac.ir/article_681_1595af6435015c77a7149e92a551338e.pdf