Arak University Cell and Tissue Journal 2228-7035 11 2 2020 06 21 Induction of Apoptosis in Human Cancer A549 Cells Through Hydroalcoholic Extract of Salvia officinalis Induction of Apoptosis in Human Cancer A549 Cells Through Hydroalcoholic Extract of Salvia officinalis 100 112 46952 10.52547/JCT.11.2.100 FA E Hoveizi Department of biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran F Pouratar Department of biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran M Kesmati Department of biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran A Shahriari Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran Journal Article 2020 11 15 <strong>Aim: </strong>The purpose of the current study is to investigate the cytotoxic and oxidative effects of Salvia officinalis hydroalcoholic extract on cancer A549 cells. <br /><strong>Material and Methods</strong>: The cells were seeded in plates to investigate concentrations of Salvia officinalis extract and MTT measurement was done to determine IC50 concentration and cell viability on days 1, 3, 5, and 7. Moreover, analyses of superoxide dismutase and catalase enzymes involved in oxidative stress pathway, and AO/EB staining for a qualitative investigation of cell lines has been conducted in order to explore the effects of IC50 concentration on apoptosis induction. Also, Giemsa and DAPI stainings were utilized to explore the morphological changes of cell and nucleus.. <br /><strong>Results:</strong> IC50 concentration of Salvia extract for A549 cells was determined 5 mg/mL. According to the results, Salvia extract reduced the cell viability of A549 cells. Based on the results, Salvia extract had a significantly greater cytotoxic effect compared to the control sample of A549 cell line. Treatment cells indicated some clear and dose-dependent differences at different times. The results of stainings proved the apoptosis induction in the treatment group. Furthermore, regarding the enzyme expression results the activity of superoxide dismutase and catalase enzymes in the cell groups treated by Salvia extract was significantly increased, compared to the control group. <br /><strong>Conclusion: </strong>Consistent with the results of this study, in addition to the anti-oxidative activity Salvia officinalis hydroalcoholic extract revealed significant apoptotic effects on lung cancer cells, considering a time and dose-dependent method. <br /><strong> </strong> <strong>Aim: </strong>The purpose of the current study is to investigate the cytotoxic and oxidative effects of Salvia officinalis hydroalcoholic extract on cancer A549 cells. <br /><strong>Material and Methods</strong>: The cells were seeded in plates to investigate concentrations of Salvia officinalis extract and MTT measurement was done to determine IC50 concentration and cell viability on days 1, 3, 5, and 7. Moreover, analyses of superoxide dismutase and catalase enzymes involved in oxidative stress pathway, and AO/EB staining for a qualitative investigation of cell lines has been conducted in order to explore the effects of IC50 concentration on apoptosis induction. Also, Giemsa and DAPI stainings were utilized to explore the morphological changes of cell and nucleus.. <br /><strong>Results:</strong> IC50 concentration of Salvia extract for A549 cells was determined 5 mg/mL. According to the results, Salvia extract reduced the cell viability of A549 cells. Based on the results, Salvia extract had a significantly greater cytotoxic effect compared to the control sample of A549 cell line. Treatment cells indicated some clear and dose-dependent differences at different times. The results of stainings proved the apoptosis induction in the treatment group. Furthermore, regarding the enzyme expression results the activity of superoxide dismutase and catalase enzymes in the cell groups treated by Salvia extract was significantly increased, compared to the control group. <br /><strong>Conclusion: </strong>Consistent with the results of this study, in addition to the anti-oxidative activity Salvia officinalis hydroalcoholic extract revealed significant apoptotic effects on lung cancer cells, considering a time and dose-dependent method. <br /><strong> </strong> https://jct.araku.ac.ir/article_46952_a36b055555e9211a3c90782b76e493d1.pdf