Arak University Cell and Tissue Journal 2228-7035 11 1 2020 03 20 Comparison of Achillea wilhelmsii, Silybum marianumseed, Echinacea purpurea, Adiantum capillus-veneris and apricot kernel extracts effects on the proliferation and apoptosis of breast cancer cells Comparison of Achillea wilhelmsii, Silybum marianumseed, Echinacea purpurea, Adiantum capillus-veneris and apricot kernel extracts effects on the proliferation and apoptosis of breast cancer cells 73 86 44768 10.52547/JCT.11.1.73 FA L Soltani Department of Animal Sciences, Faculty of Agriculture and Engineering, Razi University, Kermanshah, Iran 0000-0002-5007-5110 M Darbemamieh Department of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran 0000-0001-8610-3550 Journal Article 2020 09 12 <strong>Aim:</strong> The aim of this study was to compare the anti-proliferative and apoptotic effects of hydroalcoholic extracts of different herbal medicines (<em>Achillea wilhelmsii</em>, <em>Silybum marianum</em>seed, <em>Echinacea purpurea</em>, <em>Adiantum capillus-veneris</em>and apricot kernel) against breast cancer cells (Mcf-7). <br /><strong>Material and method:</strong> For this purpose, the plants were dried and milled, then, soaked in 70% ethanol for 72 hours and their extracts were extracted using a rotary evaporator.Different concentrations of herbal extracts (12.5, 25, 50 and 100 μg/ml) were added to the cancer cell culture medium and their cytotoxicity and apoptotic effects were investigated after 24h by MTT assay and acridine orange - ethidium bromide staining, respectively.Data was analyzed by SPSS software at the significant level of 5%. <br /><strong>Results:</strong> Addition of the highestconcentration of all extracts to the culture mediumshowed the most significant anti-proliferative and apoptotic effects (p < 0.05) compared to other concentrations of the same extracts.Also, among the high concentrations (100μg/ml), the highest cell cytotoxicity effects were related to the extracts of <em>Echinacea purpurea</em>and <em>Adiantum capillus-veneris</em> (p < 0.05). <br /><strong>Conclusion:</strong> The results of this study indicated that the addition of <em>Echinacea purpurea</em> and <em>Adiantum capillus-veneris</em>extractsto the cell culturesin high concentrationshad the most significant anti proliferative and apoptotic effects on breast cancer cells in comparison with other plant extractsand concentrations. <br /><strong> </strong> <strong>Aim:</strong> The aim of this study was to compare the anti-proliferative and apoptotic effects of hydroalcoholic extracts of different herbal medicines (<em>Achillea wilhelmsii</em>, <em>Silybum marianum</em>seed, <em>Echinacea purpurea</em>, <em>Adiantum capillus-veneris</em>and apricot kernel) against breast cancer cells (Mcf-7). <br /><strong>Material and method:</strong> For this purpose, the plants were dried and milled, then, soaked in 70% ethanol for 72 hours and their extracts were extracted using a rotary evaporator.Different concentrations of herbal extracts (12.5, 25, 50 and 100 μg/ml) were added to the cancer cell culture medium and their cytotoxicity and apoptotic effects were investigated after 24h by MTT assay and acridine orange - ethidium bromide staining, respectively.Data was analyzed by SPSS software at the significant level of 5%. <br /><strong>Results:</strong> Addition of the highestconcentration of all extracts to the culture mediumshowed the most significant anti-proliferative and apoptotic effects (p < 0.05) compared to other concentrations of the same extracts.Also, among the high concentrations (100μg/ml), the highest cell cytotoxicity effects were related to the extracts of <em>Echinacea purpurea</em>and <em>Adiantum capillus-veneris</em> (p < 0.05). <br /><strong>Conclusion:</strong> The results of this study indicated that the addition of <em>Echinacea purpurea</em> and <em>Adiantum capillus-veneris</em>extractsto the cell culturesin high concentrationshad the most significant anti proliferative and apoptotic effects on breast cancer cells in comparison with other plant extractsand concentrations. <br /><strong> </strong> https://jct.araku.ac.ir/article_44768_7629497d530cf5d916817a7e867063b3.pdf