Arak University Cell and Tissue Journal 2228-7035 4 3 2013 01 01 Bioinformatics and Molecular Cytogenetic Study of a Small Region of Chromosome 15 Including Allelic Imbalances in Uterine Cancer Prone Inice Bioinformatics and Molecular Cytogenetic Study of a Small Region of Chromosome 15 Including Allelic Imbalances in Uterine Cancer Prone Inice 297 303 4406 10.52547/JCT.4.3.297 FA Journal Article 2014 01 26 Aim: Previous studies using LOH technique on BDII rats showed four distinct Allelic imbalance on chromosome 15. In this research we have studied region 2 including Allelic imbalance by bioinformatic approachs and FISH (Florescence in situ hybridization) technique. <br />Material and Methods: The  confirmed tumors by an expert pathologist were used for cell culture. Metaphase chromosomes were prepared by conventional methods. The corresponding genes labeled probe for hybridization was poured onto slides. The labeled probe was then subjected to detected phase. Slides were studied under a fluorescence microscope using software CW4000 Laica. <br />Results: Using some of the database we could register 104 genes. But most of them did not have a clear function. According of FISH results, <em>Dlgap5</em>, <em>Fermt2</em> and Socs4 had amplification and Lgals3 had copy number reduction. <br />Conclusion: according to our results, the Dlgap5 and Socs4 are probably involve in EAC Aim: Previous studies using LOH technique on BDII rats showed four distinct Allelic imbalance on chromosome 15. In this research we have studied region 2 including Allelic imbalance by bioinformatic approachs and FISH (Florescence in situ hybridization) technique. <br />Material and Methods: The  confirmed tumors by an expert pathologist were used for cell culture. Metaphase chromosomes were prepared by conventional methods. The corresponding genes labeled probe for hybridization was poured onto slides. The labeled probe was then subjected to detected phase. Slides were studied under a fluorescence microscope using software CW4000 Laica. <br />Results: Using some of the database we could register 104 genes. But most of them did not have a clear function. According of FISH results, <em>Dlgap5</em>, <em>Fermt2</em> and Socs4 had amplification and Lgals3 had copy number reduction. <br />Conclusion: according to our results, the Dlgap5 and Socs4 are probably involve in EAC https://jct.araku.ac.ir/article_4406_ae87a54e183c075c494c4d397d126a66.pdf