Arak University Cell and Tissue Journal 2228-7035 9 4 2018 12 22 Evaluation of the protective effects of StachyslavandulifoliaVahl. on ethanol induced testicular damages in Wistarrats Evaluation of the protective effects of StachyslavandulifoliaVahl. on ethanol induced testicular damages in Wistarrats 321 332 34429 10.52547/JCT.9.4.321 FA M Mirzaei Fard Department of bioscience, Malek Ashtar University of Tehran, Tehran, Iran A Aramvash Department of bioscience, Malek Ashtar University of Tehran, Tehran, Iran Journal Article 2019 02 27 <strong>Aim</strong>: In this study, the effects of recombinant human growth hormone and Gemcitabine alone and in combination with each other were investigated on the lung fibroblast cell lines. <br /><strong>Material and methods</strong>: Human growth hormone at 10-400 ng/ml and Gemcitabine at 1-100 μg/ml concentrations were treated with the human lung fibroblast cells and the analysis were performed with MTT assay, propidium iodide staining and scratch assay. <br /><strong>Results:</strong> Studies of the cell cycle and MTT assay revealed the effect of growth hormone on the progression of the cell cycle and exit of G1 phase and the cell proliferation, and the inhibitory effects of the cell cycle and growth process by Gemcitabin compared with the control sample. The results of the scratch test indicated that the growth hormone at its effective concentrations caused increased the cell migration compared to control, while the Gemcitabin reduced the migration of the cell to the control. <br /><strong>Conclusions:</strong> Using growth hormone in combination to gemcitabin during chemotherapy treatment, it seems that we can reduce the rate of the neem normal cells death. Since these cells are more sensitive to growth hormone´s effect than cancer cells. <br />  <strong>Aim</strong>: In this study, the effects of recombinant human growth hormone and Gemcitabine alone and in combination with each other were investigated on the lung fibroblast cell lines. <br /><strong>Material and methods</strong>: Human growth hormone at 10-400 ng/ml and Gemcitabine at 1-100 μg/ml concentrations were treated with the human lung fibroblast cells and the analysis were performed with MTT assay, propidium iodide staining and scratch assay. <br /><strong>Results:</strong> Studies of the cell cycle and MTT assay revealed the effect of growth hormone on the progression of the cell cycle and exit of G1 phase and the cell proliferation, and the inhibitory effects of the cell cycle and growth process by Gemcitabin compared with the control sample. The results of the scratch test indicated that the growth hormone at its effective concentrations caused increased the cell migration compared to control, while the Gemcitabin reduced the migration of the cell to the control. <br /><strong>Conclusions:</strong> Using growth hormone in combination to gemcitabin during chemotherapy treatment, it seems that we can reduce the rate of the neem normal cells death. Since these cells are more sensitive to growth hormone´s effect than cancer cells. <br />  https://jct.araku.ac.ir/article_34429_5824aa83d1ae73b56a67273714b57843.pdf