Arak University Cell and Tissue Journal 2228-7035 9 3 2018 09 23 Beta-Aminobutyric Acid (BABA) Effect on Induced Resistance in Tomato-Infected Bacteria Pseudomonas syringae pv. Syringa Beta-Aminobutyric Acid (BABA) Effect on Induced Resistance in Tomato-Infected Bacteria Pseudomonas syringae pv. Syringa 196 205 34387 10.52547/JCT.9.3.196 FA H Pourabtahi Departeman of Biotechnology, College of Agriculture, university of Shiraz, Shiraz, Iran A Moghadam Departeman of Biotechnology, College of Agriculture, university of Shiraz, Shiraz, Iran Z Heydarian Departeman of Biotechnology, College of Agriculture, university of Shiraz, Shiraz, Iran Journal Article 2019 02 25 Aim: In this study, the pattern of gene expression (PR1, NCED and SPR2) was considered as informative markers of systemic resistance, and positive beta-amino-butyric acid (BABA) effects was investigated on induction of resistance in Lycopersicon esclentum Mill. Material and Methods: L. esclentum (Tomato) cv. Hungarian was selected in the four-leaved stage. For each pot, 70 ml of a 250 mM BABA solution was prepared and sprayed on plant leaves. The treated pots were kept under controlled conditions (16 h light at 30 ° C and 8 h darkness at 25 ° C) for 2 days, before the bacteria were induced. After two days, the bacteria were inoculated. The total RNA from leaves was extracted at 0, 24, 72, and 96 h after inoculation. The cDNA was synthesized and the gene expression pattern was determined by RT-PCR method. Results: The level of expression of three defense-related genes (PR1, NCED and SPR2) increases as a result of bacterial contamination. However, pre-treatment with BABA resulted in a significant increase of resistance gene expression in response to the challenge of pathogen relative to the control plant and the apparent symptoms of the disease (As roundish and irregular spots up to brown and black with chlorosis) causing damage to tomatoes. Conclusion: Pre-treatment of the plant with BABA has enhanced the plant's defense system, and has shown the extreme effect of BABA on plant resistance. As a result, this indictor can be used to control a P. syringae pv. syring in tomatoes Aim: In this study, the pattern of gene expression (PR1, NCED and SPR2) was considered as informative markers of systemic resistance, and positive beta-amino-butyric acid (BABA) effects was investigated on induction of resistance in Lycopersicon esclentum Mill. Material and Methods: L. esclentum (Tomato) cv. Hungarian was selected in the four-leaved stage. For each pot, 70 ml of a 250 mM BABA solution was prepared and sprayed on plant leaves. The treated pots were kept under controlled conditions (16 h light at 30 ° C and 8 h darkness at 25 ° C) for 2 days, before the bacteria were induced. After two days, the bacteria were inoculated. The total RNA from leaves was extracted at 0, 24, 72, and 96 h after inoculation. The cDNA was synthesized and the gene expression pattern was determined by RT-PCR method. Results: The level of expression of three defense-related genes (PR1, NCED and SPR2) increases as a result of bacterial contamination. However, pre-treatment with BABA resulted in a significant increase of resistance gene expression in response to the challenge of pathogen relative to the control plant and the apparent symptoms of the disease (As roundish and irregular spots up to brown and black with chlorosis) causing damage to tomatoes. Conclusion: Pre-treatment of the plant with BABA has enhanced the plant's defense system, and has shown the extreme effect of BABA on plant resistance. As a result, this indictor can be used to control a P. syringae pv. syring in tomatoes

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