Arak University Cell and Tissue Journal 2228-7035 8 4 2017 12 22 Investigating the effects of Resveratrol on proliferation and colony formation of dissociated human embryonic stem cells Investigating the effects of Resveratrol on proliferation and colony formation of dissociated human embryonic stem cells 322 331 31087 10.52547/JCT.8.4.322 FA Zahra Safaeinejad Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Mohammad Nabiuni Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran 0000-0002-0349-6676 Maryam Peymani Department of Biology, Sharekord Branch, Islamic Azad University, Sharekord, Iran. Kamran Ghaedi Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran. Mohammad Hossein Nasr-Esfahani Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran Journal Article 2018 05 15 <strong>Aim:</strong> The aim of this study was study the role of Resveratrol (RSV) on proliferation and colony efficiency of dissociated human embryonic stem cells (hESCs). <br /><strong>Material and methods</strong>: In the current study, we used HESC line, RH6. Cells were cultured on the matrigelin supplemented hESC specific medium. Cell proliferation was estimated using cell counting and Brdu incorporation assays. The expression of colony efficiency markers (E-cadherin and β-catenin) was evaluated by western blot technique and β-catenin localization examined by immune-cytochemistry staining. <br /><strong>Results:</strong> We have shown that RSV promoted hPSCs proliferation without affecting their colony efficiency. <br /><strong>Conclusion:</strong> According to these observations, RSVcan be suggested as a new supplement for hESCs culture. <br />  <br />  <strong>Aim:</strong> The aim of this study was study the role of Resveratrol (RSV) on proliferation and colony efficiency of dissociated human embryonic stem cells (hESCs). <br /><strong>Material and methods</strong>: In the current study, we used HESC line, RH6. Cells were cultured on the matrigelin supplemented hESC specific medium. Cell proliferation was estimated using cell counting and Brdu incorporation assays. The expression of colony efficiency markers (E-cadherin and β-catenin) was evaluated by western blot technique and β-catenin localization examined by immune-cytochemistry staining. <br /><strong>Results:</strong> We have shown that RSV promoted hPSCs proliferation without affecting their colony efficiency. <br /><strong>Conclusion:</strong> According to these observations, RSVcan be suggested as a new supplement for hESCs culture. <br />  <br />  https://jct.araku.ac.ir/article_31087_808c180bdf9dbb4aa16f114e795a59e8.pdf