Arak University Cell and Tissue Journal 2228-7035 8 4 2017 12 22 Effects of vitamin D on myelin basic protein expression in corpus callosum of mouse model of experimental autoimmune encephalomyelitis induced by Cuprizone Effects of vitamin D on myelin basic protein expression in corpus callosum of mouse model of experimental autoimmune encephalomyelitis induced by Cuprizone 314 321 31086 10.52547/JCT.8.4.314 FA Farhad Mashayekhi Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran 0000-0002-4226-2426 Zivar Salehi Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran Ebrahim Mirzajani Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran Journal Article 2018 05 15 Aims: In this study, the effects of Vitamin D3 on total protein concentration (TPC) and MBP expression in the corpus callosum extracts of Cuprizone induced demyelinated mouse has been investigated. Material and Methods: The mice were treated by Cuprizone for five weeks in order to induce demyelination. Then, the mice were divided into 3 groups. The first group was injected intraperitoneally (IP) by vitamin D3 in the amount of 5 µg/kg/daily body weight. The second group (SHAM) was injected IP by phosphate buffered saline (PBS) and the third group was left without injection as controls. After five weeks, the mice were killed and the corpus callosum was collected and the total protein concentration (TPC) and expression of MBP were studied by Western blot technique. Results: No significant variation in the cortical TPC was seen in vitamin D3 injected group as compared to either SHAM or controls. We have also shown that the expression of MBP in the corpus callosum extracts of demyelinated mouse was significantly increased in the vitamin D3 injected group as compared to the other groups. Conclusions: It is concluded that vitamin D3 increases MBP expression in the corpus callosum of cuprizone-induced demyelination mice. It is also suggested that vitamin D3 may play a role in the process of remyelination by increasing MBP expression in the cortex. Aims: In this study, the effects of Vitamin D3 on total protein concentration (TPC) and MBP expression in the corpus callosum extracts of Cuprizone induced demyelinated mouse has been investigated. Material and Methods: The mice were treated by Cuprizone for five weeks in order to induce demyelination. Then, the mice were divided into 3 groups. The first group was injected intraperitoneally (IP) by vitamin D3 in the amount of 5 µg/kg/daily body weight. The second group (SHAM) was injected IP by phosphate buffered saline (PBS) and the third group was left without injection as controls. After five weeks, the mice were killed and the corpus callosum was collected and the total protein concentration (TPC) and expression of MBP were studied by Western blot technique. Results: No significant variation in the cortical TPC was seen in vitamin D3 injected group as compared to either SHAM or controls. We have also shown that the expression of MBP in the corpus callosum extracts of demyelinated mouse was significantly increased in the vitamin D3 injected group as compared to the other groups. Conclusions: It is concluded that vitamin D3 increases MBP expression in the corpus callosum of cuprizone-induced demyelination mice. It is also suggested that vitamin D3 may play a role in the process of remyelination by increasing MBP expression in the cortex.

https://jct.araku.ac.ir/article_31086_49dee2008d36b4304fcb72e867f40c14.pdf