Arak University Cell and Tissue Journal 2228-7035 11 4 2021 02 19 The effect of ethanolic extract of Achilleawilhelmsii on the survival of three cancer cell lines in vitro culture The effect of ethanolic extract of Achilleawilhelmsii on the survival of three cancer cell lines in vitro culture 302 311 244131 10.52547/JCT.11.4.302 FA F Khosravi Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran J Moshtaghian Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran SH Zarkesh Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran Journal Article 2021 05 22 <strong>Aim: </strong>This experiment was performed to investigate the effect of ethanolic extract of yarrow leaves and flowers on the survival of three cancer cell lines namely; HELA, CAOV, MCF7, for the possibile production of inexpensive drugs without side effects. <br /><strong>Material and Methods</strong>: After incubation of cancer cell liners 48 hours, cell suspension was prepared and distributed with cells at different concentration in culture medium for 24 hours. Then <em>Achilleawilhelmsii</em> leaf and flower extract were prepared and sterilized. Consecutive dilutions of 0.4, 0.8, 1.6, 3.2 and 6.4 mg / ml were prepared and added to each well containing cell suspension and placed in an incubator. The plates were examined microscopically after 48 hours and light absorption was determined after MTT assay. <br /><strong>Results: </strong>The results showed that in MCF7 with 0.4 mg / ml; CAOV, and HELA cancer cell lines, the lowest cell survival rate was observed at the concentration of 6.4 mg / ml leaf extraction. In comparison, the highest anti-cancer effect of yarrow flower extract on three cancer cell lines was recorded in MCF7 cancer cells at a concentration of 6.4 mg / ml, while in HELA cancer cell line the lowest cell survival ware observed at the concentration of 6.4 mg / ml in a 72-hour treatment. <br /><strong>Conclusion</strong>: According to the results, yarrow flower and leaf extract treatment is recommended to decrease of three cancer cell lines survival. <br />  <br /><strong> </strong> <strong>Aim: </strong>This experiment was performed to investigate the effect of ethanolic extract of yarrow leaves and flowers on the survival of three cancer cell lines namely; HELA, CAOV, MCF7, for the possibile production of inexpensive drugs without side effects. <br /><strong>Material and Methods</strong>: After incubation of cancer cell liners 48 hours, cell suspension was prepared and distributed with cells at different concentration in culture medium for 24 hours. Then <em>Achilleawilhelmsii</em> leaf and flower extract were prepared and sterilized. Consecutive dilutions of 0.4, 0.8, 1.6, 3.2 and 6.4 mg / ml were prepared and added to each well containing cell suspension and placed in an incubator. The plates were examined microscopically after 48 hours and light absorption was determined after MTT assay. <br /><strong>Results: </strong>The results showed that in MCF7 with 0.4 mg / ml; CAOV, and HELA cancer cell lines, the lowest cell survival rate was observed at the concentration of 6.4 mg / ml leaf extraction. In comparison, the highest anti-cancer effect of yarrow flower extract on three cancer cell lines was recorded in MCF7 cancer cells at a concentration of 6.4 mg / ml, while in HELA cancer cell line the lowest cell survival ware observed at the concentration of 6.4 mg / ml in a 72-hour treatment. <br /><strong>Conclusion</strong>: According to the results, yarrow flower and leaf extract treatment is recommended to decrease of three cancer cell lines survival. <br />  <br /><strong> </strong> https://jct.araku.ac.ir/article_244131_644a28d23a35523e1b795e40aa77ce1a.pdf