Arak University Cell and Tissue Journal 2228-7035 7 3 2016 09 22 Cytocidal effects of Bacillus thuringiensis crystal protein on mice breast cancer cell line in in vitro condition Cytocidal effects of Bacillus thuringiensis crystal protein on mice breast cancer cell line in in vitro condition 243 250 23810 10.52547/JCT.7.3.243 FA SH Shekufe Biazar دانشجوی کارشناسی ارشد دانشگاه آزاد اسلامی، واحد علوم و تحقیقات فارس، دانشکده علوم پایه، گروه میکروب‌شناسی، شیراز، ایران E Moazamian دانشگاه آزاد اسلامی، واحد شیراز، گروه میکروب‌شناسی، شیراز، ایران N Azarpira دانشگاه علوم پزشکی شیراز، مرکز تحقیقات پیوند و ترمیم اعضا، شیراز، ایران Journal Article 2017 01 22 <strong>Aim:</strong>In the study, the effects of <em>Bacillus</em> <em>thuringiensis</em> parasporin were examined on mice cancer cell line (4T1). <br /><strong>Material and Methods:</strong> Purification of crystal toxins was done from the isolates of <em>B.</em> <em>thuringiensis</em>. Breast cancer cells line 4T1 were treated with activated toxins. Cytopathic effects were photographed. Crystal toxin with the most cytotoxicity was identified by using SDS-PAGE and also molecular method. <br /><strong>Results: </strong>From forty seven tested isolates of <em>B.</em> <em>thuringiensis</em>, E8 isolate shown the most cytotoxicity effect on 4T1 cell line. In addition, parasporin-4 was identified by using SDS-PAGE and PCR methods. Parasporin-4 disintegrated 4T1 cell line and it has cytolysin effects. <br /><strong>Conclusion</strong>: Our data suggest that, parasporin is cytolysin protein and has a specific site on the cell surface. This crystal protein can induce apoptosis in breast cancer cell line. <strong>Aim:</strong>In the study, the effects of <em>Bacillus</em> <em>thuringiensis</em> parasporin were examined on mice cancer cell line (4T1). <br /><strong>Material and Methods:</strong> Purification of crystal toxins was done from the isolates of <em>B.</em> <em>thuringiensis</em>. Breast cancer cells line 4T1 were treated with activated toxins. Cytopathic effects were photographed. Crystal toxin with the most cytotoxicity was identified by using SDS-PAGE and also molecular method. <br /><strong>Results: </strong>From forty seven tested isolates of <em>B.</em> <em>thuringiensis</em>, E8 isolate shown the most cytotoxicity effect on 4T1 cell line. In addition, parasporin-4 was identified by using SDS-PAGE and PCR methods. Parasporin-4 disintegrated 4T1 cell line and it has cytolysin effects. <br /><strong>Conclusion</strong>: Our data suggest that, parasporin is cytolysin protein and has a specific site on the cell surface. This crystal protein can induce apoptosis in breast cancer cell line. https://jct.araku.ac.ir/article_23810_63168d42036e611d664ef366dc1bf40e.pdf