Arak University Cell and Tissue Journal 2228-7035 6 3 2016 02 01 Investigating the Effects of Hydrocortisone Hormone on Induced Clastogenical Chromosomal Abnormalities on L929 Cell Line Using Micronucleus Assay on Binucleated Cells Investigating the Effects of Hydrocortisone Hormone on Induced Clastogenical Chromosomal Abnormalities on L929 Cell Line Using Micronucleus Assay on Binucleated Cells 249 256 18740 10.52547/JCT.6.3.249 FA Y S F H 0000-0002-5998-7183 M M 0000-0002-7949-7712 SH S H A Journal Article 2016 02 24 <strong>Aim:</strong> In this study the effects of stress on induction of structural chromosomal abnormalities on L929 cell line using micronucleus assay on cytokinesis-blocked binucleated cells were investigated <em>in vitro</em>. <strong>Material and methods:</strong> L929 cells were cultured in DMEM containing 10% FBS. The cells were divided into four groups including; control, 2Gy gamma-irradiated cells, and cells treated with doses of 25, 50 and 100 µg/ml hydrocortisone, and cells co-treated with these three doses of hydrocortisone and irradiation. The treated cells were harvested, stained and chromosome abnormalities were scored using micronucleus assay. <strong>Results:</strong> Results showed that hydrocortisone did not induce micronuclei as compared to the control group. However, the frequency of micronuclei in cells co-treated with doses of hydrocortisone and irradiation was significantly higher than cells treated only with gamma irradiation (p < 0.05). <strong>Conclusion:</strong> According to the data of this study, stress hormones are not able to induce any chromosomal abnormalities; however, they are able to increase the cell susceptibility to clastogenic effects of irradiation. <strong>Key words:</strong> Stress, Hydrocortisone, L929, Gamma ray, Micronucleus <strong> </strong> <strong> </strong> <strong> </strong> <strong>Aim:</strong> In this study the effects of stress on induction of structural chromosomal abnormalities on L929 cell line using micronucleus assay on cytokinesis-blocked binucleated cells were investigated <em>in vitro</em>. <strong>Material and methods:</strong> L929 cells were cultured in DMEM containing 10% FBS. The cells were divided into four groups including; control, 2Gy gamma-irradiated cells, and cells treated with doses of 25, 50 and 100 µg/ml hydrocortisone, and cells co-treated with these three doses of hydrocortisone and irradiation. The treated cells were harvested, stained and chromosome abnormalities were scored using micronucleus assay. <strong>Results:</strong> Results showed that hydrocortisone did not induce micronuclei as compared to the control group. However, the frequency of micronuclei in cells co-treated with doses of hydrocortisone and irradiation was significantly higher than cells treated only with gamma irradiation (p < 0.05). <strong>Conclusion:</strong> According to the data of this study, stress hormones are not able to induce any chromosomal abnormalities; however, they are able to increase the cell susceptibility to clastogenic effects of irradiation. <strong>Key words:</strong> Stress, Hydrocortisone, L929, Gamma ray, Micronucleus <strong> </strong> <strong> </strong> <strong> </strong> https://jct.araku.ac.ir/article_18740_705c03a1245566a3edb2d1c3ddcbb6ff.pdf