Arak University Cell and Tissue Journal 2228-7035 6 1 2015 04 01 Survival Potential Investigation of the Adipose-Derived Mesenchymal Stem Cell in the Natural Scaffolds as a Suitable Growth Medium Survival Potential Investigation of the Adipose-Derived Mesenchymal Stem Cell in the Natural Scaffolds as a Suitable Growth Medium 23 29 12476 10.52547/JCT.6.1.23 FA M Gh R T N K 0000-0001-8870-8035 M M M Sh Journal Article 2015 04 22 Aim: In this study, it was done to evaluate the efficiency of both PRP and Fibrin Glue scaffolds in producing suitable environment for the growth of mesenchymal stem cells. <br />Material and Methods: In this study, the preparation of PRP and Fibrin Glue Scaffold were carry out and Mesenchymal Stem Cells (MSCs) isolated from adipose tissue. The mesenchymal phenotype of these cells was determined by mesenchymal surface marker using flow cytometry. Then, MSCs were cultured, and on the third passage (P3)stage, they were seeded separately on the two scaffolds and after 48 hours of cell culture, the ability of the scaffolds seeded cells was evaluated by MTT assay for cells viability. <br />Results:Flow cytometry results showed that human adipose-derived mesenchymal stem cell expressed CD44, CD90 and CD105 surface markers. Also, the results of this study showed that the active PRP could be creating a more suitable environment for the survival and proliferation of mesenchymal stem cells in compare with Fibrin glue <br />Conclusion:It is suggested that the PRP as a protective scaffold could be used for mesenchymal stem cells growth to provide effective strategies in order to tissue engineering development and regenerative medicine. Aim: In this study, it was done to evaluate the efficiency of both PRP and Fibrin Glue scaffolds in producing suitable environment for the growth of mesenchymal stem cells. <br />Material and Methods: In this study, the preparation of PRP and Fibrin Glue Scaffold were carry out and Mesenchymal Stem Cells (MSCs) isolated from adipose tissue. The mesenchymal phenotype of these cells was determined by mesenchymal surface marker using flow cytometry. Then, MSCs were cultured, and on the third passage (P3)stage, they were seeded separately on the two scaffolds and after 48 hours of cell culture, the ability of the scaffolds seeded cells was evaluated by MTT assay for cells viability. <br />Results:Flow cytometry results showed that human adipose-derived mesenchymal stem cell expressed CD44, CD90 and CD105 surface markers. Also, the results of this study showed that the active PRP could be creating a more suitable environment for the survival and proliferation of mesenchymal stem cells in compare with Fibrin glue <br />Conclusion:It is suggested that the PRP as a protective scaffold could be used for mesenchymal stem cells growth to provide effective strategies in order to tissue engineering development and regenerative medicine. https://jct.araku.ac.ir/article_12476_4f6efc6c35919c6eae28fdcf790fc974.pdf