Evaluation of Auxin (IAA) and Kinetin Effects on Lead Uptake and Accumulation in Matthiola flavida Bioss
Volume 3, Issue 2, Winter 2012, Pages 161-169
https://doi.org/10.52547/JCT.3.2.161
Abstract Aim: The aim of present study was evaluation of IAA and kinetin effects on lead uptake and accumulation in Matthiola flavida.
Material and methods: Metallicolous population of M. flavida was grown in hydroponic condition. Then plants were exposed to combination of 1µM Pb and 0, 1, 10, 100 µM IAA and kinetin for 14 days. Finally, Pb concentration was determined in their aerial parts and roots using a flam atomic absorption. Also their Chlorophyll contents were measured and calculated by spectrophotometer.
Results: Results showed increasing aerial part Pb concentration more than 48 and 110% respectively in 1 and 10 µM IAA comparing to control. A significant decreasing aerial part Pb concentration was observed with increasing of kinetin concentration in culture medium. Root Pb concentration significantly decreased with increasing of auxin and kinetin in nutrient solution. Significant differences in aerial part dry weight were not observed in different IAA and kinetin concentrations in comprising with control.
Conclusion: Increasing aerial part Pb concentration in M. flavida was observed in low concentration of IAA but kinetin did not show clear and positive effects on biomass and Pb concentration.