Cell and Tissue Journal

Abstract Aim: Appendicitis is one of the most common abdominal inflammatory diseases. The function of appendix is not clearly defined. In this study to understand the immunological function of the appendix, we investigated the function and phenotypic pattern of lymphocyte in appendix of patients with normal and inflamed appendix tissue.
Material and Methods: Appendix tissue and appendiceal mononuclear cells obtained from 81 patients (mean age; 23±10.5), who was clinicallysuspected of having appendicitis were collected. Based on histopathological examination, twenty-five patients had normal appendix while 40 and 16 were diagnosed with suppurative and gangrene appendicitis, respectively. The phenotypic characteristics of lymphocyte subsets in appendix was analyzed using three color-flow cytometry. In addition, the proliferative response of tissue mononuclear cells was assayed by MTT method.
Results: A significant difference (p < 0. 01) was observed  in the percentage of CD19/HLA-DR, HLA-DR and CD19 cellsfrom patients suffering from suppurative appendicitis in comparison with the patients having normal appendix or gangrene one.  There was also a significant difference (p < 0.01) in the proliferative responses of the appendiceal mononuclear cells to PHA and LPS from pathients with suppurative appendicitis when compare with other groups.
Conclusion: The phenotypic and function of lymphocytes are different between normal and inflamed appendix tissue. These results showed that the appendix tissue with special lymphocyte profile may be effective in preventing or reducing intestinal infections.
 

Abstract Aim: Researchers have made considerable efforts to determine the significance of particular ABO antigens to diseases susceptibility such as cancer and infections. O blood group people have a higher risk than other blood groups for catching cholera. Thus relationship between ABO blood groups and catching disease is unknown remained. The aim of this study is to determine the lymphocyte phenotypic profile in ABO blood groups.
Material and Methods: Peripheral blood samples collected from forty health male people with different ABO blood groups (each group=10). All of studied people were in 18-25 years old ranges and had a closely similar genetic background. The samples were studied using a FACSort flow cytometr for determination of CD3+T-lymphocytes phenotype and their subsets CD4+, CD8+ and Treg cells (CD4+/CD25+/Foxp3+), CD19+ B-lymphocytes and their subsets CD5+ and CD5‾ B cells.
Results: There were no significant differences in the frequency of examined lymphocyte subpopulations (CD8+ and Treg lymphocytes, and B- lymphocyte subsets) between the ABO blood groups. But, the CD4+ T lymphocytes percentage in B blood group was higher than other groups (p < /em>=0.05).
Conclusion: High frequency of CD4+T lymphocytes in B blood group may be reduced catching some disease such as bacterial and parasitic infections.