Keywords = Guaiacol peroxidase

The effect of cadmium toxicity on health and risk index, coexistence and activity of some coriander antioxidant enzymes inoculated with Mycorrhiza fungi

Volume 11, Issue 1, Summer 2020, Pages 55-72

https://doi.org/10.52547/JCT.11.1.55

F Mohammadifard, M Moghaddam

Abstract Aim: The aim of this study was to investigate the effects of inoculation of two species of mycorrhizal fungi on growth, colonization percentage, antioxidant enzymes activity, health and risk index of coriander under cadmium stress.
Material and methods: A pot experiment was conducted in a completely randomized design with 2 factors and 3 replications in the research greenhouse of the Department of Horticultural Science and Landscape Engineering at the Ferdowsi University of Mashhad in 2018. The first factor was cadmium nitrate at 4 levels of 0, 20, 40, and 80 mg kg-1 soil and the second factor was mycorrhiza application at 3 levels of non-inoculation, inoculation with Glomus mosseae and Glomus intraradices.
Results: The results showed that with increasing cadmium concentration in the soil, the shoot and total biomass of plant, leaf area, number of seeds, 1000 seeds weight, colonization percentage, soluble protein, catalase, ascorbate peroxidase, guaiacol peroxidase, and polyphenol oxidase activities were significantly decreased in coriander. But malondialdehyde content, health and risk index increased. However, the use of mycorrhizal fungi reduced the harmful effects of cadmium in the plant. This resulted in a decrease of 47.1% of the risk and health index and 17.2% of malondialdehyde in the plant.
Conclusion: According to the findings, use of mycorrhizal fungi had a significant effect on the improving of harmful effects of cadmium in coriander, thus improved the risk and health index for consumers. Therefore, using mycorrhizal fungi as a management strategy in polluted areas with this heavy metal is 

The interaction effect of salinity stress and superabsorbent polymer on antioxidant enzyme activities of basil

Volume 9, Issue 3, Winter 2019, Pages 222-237

https://doi.org/10.52547/JCT.9.3.222

S Farsari, M Moghaddam

Abstract Aim: The aim of this study is to evaluate the interaction effect of salinity stress and superabsorbent polymers on physiological traits of basil.
Material and methods: A pot experiment was conducted as factorial based on a completely randomized design with four levels of salinity (0, 40, 80 and 120 mM NaCl in irrigation water) and four levels of superabsorbent polymers included (control, Ackoasorb, Stockosorb and Terracottem). The measured traits were soluble protein, antioxidant enzyme activities, malondialdehyde content and essential oil production.
Results: Antioxidant enzyme activities of catalase, guaiacol peroxidase, ascorbate peroxidase and polyphenol oxidase at first harvesting and high salinity (120 mM) under Ackoasorb, Terracottem, Terracottem and Ackoasorb usage decreased 52.68, 73.1, 68.07 and 75.35%, respectively, and also at second harvest at highest salinity level (80 mM) the activity of these enzymes under Ackoasorb, Terracottem, Ackoasorb, Terracottem and Terracottem decreased 37.6, 62.5, 46.38 43.06 and 38.47%, respectively.  With increasing salinity the essential oil production decreased and Tracheotem superabsorbent increase it. At first harvesting, a significant positive correlation was observed between malondialdehyde with guaiacol peroxidase (r = 0.751) and at second harvesting ascorbate peroxidase with protein (r = - 0.753) had a significant negative correlations, moreover, significant positive correlations were found between superoxide dismutase with guaiacol peroxidase (r = 0.848), malondialdehyde with guaiacol peroxidase (r = 0.789) and malondialdehyde with ascorbate peroxidase (r = 0.743).
Conclusion: The results showed that application of superabsorbents under salinity stress conditions could decrease the severity of this stress and thereby cause to decrease the antioxidant enzyme activities and malondialdehyde amount significantly, but increased the soluble protein and essential oil content.
 

Changes in protein and antioxidant system of Cucumis sativus cv. Isfahani in response to drought stress

Volume 7, Issue 4, Spring 2017, Pages 375-385

https://doi.org/10.52547/JCT.7.4.375

i F Amin, M Askari, M Haghir

Abstract Aim: Iran is considered as an arid and semi-arid area of the world and also it is one of the main countries producing cucumbers; so study the effects of drought on this plant and also its antioxidant system changes are important.
Material and methods: In this study, cucumber cv. Isfahani plants were grown under greenhouse condition. In order to study the effect of changes in protein and antioxidant systems, cucumber plants at 3 to 5 leaf stage were treated with 4 levels of drought stress (100, 75, 50 and 25 percent) and their responses to applied stresses were investigated. The amounts of electrolyte leakage, lipid peroxidation, protein, proline, total antioxidant, catalase and guaiacol peroxidase activities, α-tocopherol and hydrogen peroxide were measured.
Results: The results study showed that the contents of electrolyte leakage, lipid peroxidation, proline, total antioxidant, catalase, guaiacol peroxidase, α-tocopherol and hydrogen peroxide increased under drought stresses as compared to the control plants. However, protein index decreased under drought stresses in comparison with controls.
Conclusion: The obtained findings showed that in this cultivar of cucumber tolerance to drought was related to enzymatic and non-enzymatic antioxidant activities and also proline accumulation.