Cell and Tissue Journal

Abstract Aim: This investigation was conducted to determine the amount of isolated anthocyanins from seed coats of Phaseolus vulgaris L. under magnetic field treatment and also assess their antiproliferative activity of the extracts.
Material and method: Dry and soaked red bean seeds were subjected to different electromagnetic field treatments in four group. The seeds of groups 1(dry) and 2 (soaked) were treated for 45 min., while in groups 3 and 4 the dry and soaked seeds treated two times with 120 min. interval with electromagnetic field.   Then, they were cultured and grown, and their produced seeds were collected. Seed coats from these beans were separated and coat extract was prepared. Two groups of anthocyanins, cyanidin and pelargonidin, were identified and measured by high performance liquid chromatography (HPLC). The antiproliferative activity of the extract was determined by MTT assay against ovarian cancer cells (CP2780A).
Results: The bean coats of the control and the treated plants contain a high content of cyanidin and pelargonidin. Among the extracts, the samples of group 1 had the highest amount of cyanidin, while seeds of group 2 had the highest amount of pelargonidin. The results of the antiproliferative activity revealed that the methanol extract of the treated red beans under 4 mT were highly antiproliferative activity of the cell (78.74 to 84.44 %). Moreover, the seeds of group 2 showed the highest antiproliferative activity (IC₅₀: 72.63 ± 2.2).
Conclusion: The electromagnetic field treatment is the most effective in increasing the amounts of anthocyanins in red beans, and that the red bean coat can be used as a natural source of nutrition and drug supplement with antiproliferative properties.