Cell and Tissue Journal

Abstract Aim: The aim of this study was to investigate the effece of mare serum and fetal bovine serum (FBS) on meiotic and fertilization capacity of sheep oocytes in vitro
Material and Methods: Cumulus Oocyte Complexes (COCs) were recovered from ovaries using slicing method. COC’s were washed three time in maturation medium without any serum supplementation. The COC’s were randomly divided into three groups. Group 1 (n = 170) COC’s were fresh control and cultured in maturation medium without serum supplementation. Group 2 (n = 169) COC’s were cultured in maturation medium supplemented with 10% mare serum. Group 3 (n = 167) COC’s were cultured in maturation medium supplemented with 10% FBS. Cumulus expansion was the index of oocyte maturation. To fertilize the mature oocytes, 24 h after maturation oocytes were transferred to the fertilization medium and then sperm was added to the fertilization medium. Twenty four hours post-fertilization, oocytes were denuded mechanically by gentle pipeting, fixed in a mixture of acetic acid and alcohol, stained for 10 min with 1% (w/v) orcein in 45% acetic acid and examined for their fertilization potential.
Results: The rate of oocyte maturation in groups 1, 2 and 3 were 59/82%, 77/55% and 89/27%, respectively. The rate of fertilization in three groups was 19/19%, 39/64% and 50%, respectively. Significant difference was observed between three groups (p < 0.05).
Conclusion: The results showed that the medium contain 10% percent fetal bovine have higher in vitro maturation and fertilization of oocyte rates than mare serum and the control groups

Abstract Aim: The aim of the present study was to compare quantitative distribution and histological characteristics of Paneth cells of small intestine in sheep and goats.
Material and Methods: Ten healthy adult sheep and 10 healthy adult goats (5 male and 5 female in both sexes) of the Lori Bakhtyari breed were selected from Shahrekord abattoir. Prepared tissue sections were analyzed at both light and electron microscopic levels.
Results: Paneth cells were distributed in the crypts of Liberkühn, theirs granules were larger and more numerous in deep and cells number showed an increase the proximal of the deudenum towards the distal part of the jujenum followed by a decrease from the proximal of the ileum towards its distal part. In all regions, Paneth cells were more numerous in goats than to sheep. In electron microscopic level, dense granules were found in the apical cytoplasm. Numerical density and surface microvilli of granules were greatest in the jejunum and lowest in duodenum and ileum. In all three regions of jejenum, area of cytoplasmic granules was significantly greater in sheep than in goats.
Conclusion: The result showed that paneth cellular structure is almost similar between two species in light and electron microscope levels.