The Effect of Medium, Carbon Source, Light Spectrum and Style Treatment of Auxin on Shoot and Root Regeneration of Gisela 6 Root Stock
Volume 4, Issue 2, Autumn 2013, Pages 169-185
https://doi.org/10.52547/JCT.4.2.169
Abstract Aim: In order to localize and optimize of micro propagation of Gisela 6, a valuable rootstock, the effects of medium, carbon sources, light and method of auxin application on shooting and rooting were investigated. Material and methods: The effect of six media, five carbon sources and three light spectra plus different concentrations of BA and IBA on growth characters including shoot regeneration and their length, were studied using lateral buds as explants.. Percentage of rooting, number and length of roots were surveyed in two methods: 1) pulsing method using IBA and NAA at 1.0 g.L-1 for different times; and 2) shoot planting on solid medium containing 6 concentrations of IBA and NAA independently. Results: MS was the most suitable medium and the highest and longest shootlet numbers were produced on medium containing 30 gr. L-1 of market sugar and sucrose respectively. White and red light are more effective on shoot regeneration and shoot length respectively. The highest root induction was occurred in pulsing method in presence of 1 g. L-1 of IBA. Conclusion: Optimized shoot propagation was observed on MS medium containing market sugar and BAP under white light, and optimized root induction was occurred in pulsing method in presence of IBA