Cell and Tissue Journal

Abstract Aim: This experiment was performed to investigate the effect of ethanolic extract of yarrow leaves and flowers on the survival of three cancer cell lines namely; HELA, CAOV, MCF7, for the possibile production of inexpensive drugs without side effects.
Material and Methods: After incubation of cancer cell liners 48 hours, cell suspension was prepared and distributed with cells at different concentration in culture medium for 24 hours. Then Achilleawilhelmsii leaf and flower extract were prepared and sterilized. Consecutive dilutions of 0.4, 0.8, 1.6, 3.2 and 6.4 mg / ml were prepared and added to each well containing cell suspension and placed in an incubator. The plates were examined microscopically after 48 hours and light absorption was determined after MTT assay.
Results: The results showed that in MCF7 with 0.4 mg / ml; CAOV, and HELA cancer cell lines, the lowest cell survival rate was observed at the concentration of 6.4 mg / ml leaf extraction. In comparison, the highest anti-cancer effect of yarrow flower extract on three cancer cell lines was recorded in MCF7 cancer cells at a concentration of 6.4 mg / ml, while in HELA cancer cell line the lowest cell survival ware observed at the concentration of 6.4 mg / ml in a 72-hour treatment.
Conclusion: According to the results, yarrow flower and leaf extract treatment is recommended to decrease of three cancer cell lines survival.
 
 

Abstract Aims: This study was aimed to investigate the anti-proliferative effects of hydroalcoholic extract of Blepharis persica seed and its synergy effect with doxorubicin on human breast cancer and prostate cancer cell lines.
Materials and Methods: hydroalcoholic extract of  seed was prepared using maceration, and ethanol­%70. Eight concentrations of extract and four concentrations of combined with doxorubicin (125, 62.5ngr/ml) and extract (0.625, 0.315­mg/ml) were prepared. MCF7, LNCaP and SKM cell lines were cultured .Cell­­ viability was evaluated by MTT assay after 24­h. To show apoptosis inductionof breast and prostate­ cancer cell death by extracts, Annexin/PI test were performed. BCL₂ gene expression was analyzed using Real-Time RT-qPCRfor 24,48h.
Results: The highest effects of growth inhibition were observed in the prostate, breast and fibroblast cell lines with extract, respectively. The combined effect of doxorubicin with extract in three cell lines in comparison with control did not show any significant difference. The results of Annexin / PI indicated that the percentage of initial apoptosis, delayed  apoptosis and necrosis in treated cells increased compared to control. BCL₂ expression in cell lines decreased significantly over 24 and 48 h compares to control(p < 0.01).
Conclusion: hydroalcoholic extract of B.persica seed has ability to inhibit the proliferation of cancer cell lines as well as the induction of apoptosis in cancer cells, compared with using it with doxorubicin, although the extract did not have any synergy effect with doxorubicin at lower concentrations, it can be a substituted for this kind of drug with fewer side effects.
 

Abstract Aim: In the current study, CM anticancer function of the MCF7 cells was investigated in vitro.
Material and Methods: Adipose stem cells were extracted from the cesarean women’s abdominal fat with their written informed consent at the Velayat hospital, Damghan. CM was prepared for fourth passage of hASCs that was cultured in serum-free medium for 72h. MCF7 cells were exposed to CM for 24 and 48 hours. Then cell proliferation rate, survival and apoptotic gene expression were determined using MTT, cell counting (hemocytometer) and RT-PCR.
Results: CM-treated MCF7 for 24 and 48h, showed a significant decrement in cell proliferation and viability as compared to the cells cultured in medium containing serum (control). In addition, caspase3 gene expression of CM-induced cells was increased significantly at 24 and 48h as compared to the control group. While, CM-treated cells showed a significant increment of caspase9 gene expression after 48h induction.
Conclusion: It was concluded that CM induced apoptosis by activation of caspases and reduced proliferation rate and survival of MCF7 cancer cells. Therefore, it seems that conditioned medium can be used as a supplement with other anti-cancer therapies.