Cell and Tissue Journal

Abstract Aim: This study aimed to access the rapid and improved propagation protocol production of Zamifolia plant using tissue culture.
Material and Methods: For callus formation and regeneration, two independent factorial experiments were carried out in a completely randomized design with 3 replications in the tissue culture laboratory of the National Research Institute of Flowers and Ornamental Plants in 2018. In the first experiment, to induce callogenesis, 2,4-D (0, 1, 2, and 4 mg/l) and BA (0, 1 and 2 mg/l) were used. In the second experiment, or regeneration of explants, NAA (0 and 0.5 mg/l) and BA (0, 0.5, and 1 mg/l) were applied.
Results: The results of the callogenesis experiment showed that a modified basal MS medium with concentration of 2 mg/l BA and 1 mg /l 2,4-D produced the best callus with 85% regeneration. At the regeneration stage of the callus, 0.5 mg/l BA and 0.5 mg NAA produced the highest number of seedlings and regenerated tubers (90%). Subsequently, single tuber seedlings were successfully adapted to 80%-90% in the cocopeat and perlite substrates.
Conclusion: The results were successful and could be possible to achieve the callogenesis and regeneration of Zamifolia plant with the formula presented and recommend as practical a protocol for commercial micropropagation of this valuable ornamental plant.
 

Abstract Aim: In this study, the effect of IAA on salt tolerance of tobacco plants (Nicotiana plumbaginifolia) was investigated under in vitro culture conditions.
Material and Methods: Sterile seeds of N. plumbaginifolia were cultured in MS medium. In vitro propagated seedlings under tissue culture conditions were treated with IAA (mg / l) and salinity (0, 100 and 200 mM) for 4 weeks. Physiological and biochemical parameters including H2O2, chlorophyll, soluble carbohydrate, protein and antioxidant enzyme activity were measured.
Results: Salt stress increased H2O2, proline, catalase, superoxide dismutase and ascorbate peroxidase, while reverse patterns were observed for photosynthetic pigments, soluble carbohydrates and also total protein.  In salt-treated seedlings, application of auxin increased proline, catalase and H2O2, but decreased photosynthetic pigmentation, soluble carbohydrates, total protein and some enzymes such as superoxide dismutase and ascorbate peroxidase.
Conclusion: Auxin treatment in salt-treated tobacco seedlings improved salinity tolerance by changes in some physiological parameters.
 
 

Abstract Aim: Milk thistle (Silybum marianumL.), belonging to Asteraceae as an Iranian native-landscape plant, has a well-known position in traditional medicine. But, its seeds are dormant. The current study investigated the most efficient hormonal treatment to break the species dormancy.
Material and Methods: The seeds were treated using gibberellic acid, kinetin and auxin (100, 250, 500 and 1000 ppm) –and 24-epibrassinolid (0.5 and 1 ppm) for 24 and 48 h. Also, a combined treatment of gibberellic acid (500 ppm) and pre-chilling (for 5, 10 and 15 d) was accomplished.
Results: All treatments significantly increased seed germination in milk thistle; though the most effective treatment was gibberellic acid (500 ppm) which caused 70% increasing in seed germination. Kinetin and auxin (100 ppm) and 24-epibrassinolid (0.5 ppm), in 24 h respectively increased seed germination about, 40%, 38% and 60%. Also combination of gibberellic acid (500 ppm) and chilling (5d) treatment increased seed germinating 60%.
Conclusion: Results showed gibberellic acid (500 ppm) is the best treatment for breaking of Silybum marianum seed dormancy.
 

Abstract Aim: The aim of present study was evaluation of IAA and kinetin effects on lead uptake and accumulation in Matthiola flavida.
Material and methods: Metallicolous population of M. flavida was grown in hydroponic condition. Then plants were exposed to combination of 1µM Pb and 0, 1, 10, 100 µM IAA and kinetin for 14 days. Finally, Pb concentration was determined in their aerial parts and roots using a flam atomic absorption. Also their Chlorophyll contents were measured and calculated by spectrophotometer.
Results: Results showed increasing aerial part Pb concentration more than 48 and 110% respectively in 1 and 10 µM IAA comparing to control. A significant decreasing aerial part Pb concentration was observed with increasing of kinetin concentration in culture medium. Root Pb concentration significantly decreased with increasing of auxin and kinetin in nutrient solution. Significant differences in aerial part dry weight were not observed in different IAA and kinetin concentrations in comprising with control.
Conclusion: Increasing aerial part Pb concentration in M. flavida was observed in low concentration of IAA but kinetin did not show clear and positive effects on biomass and Pb concentration.
 

Abstract < p >Aim: The aim of this research is evaluation of auxin changes in plant regenerated from tobacco root with Ri-TDNA Material and methods: In this research leaf segments of tobacco were transformed by Agrobacterium rhizogenes. Transformed hairy root was selected by their ability to grow on medium containing Kanamycin. For confirmation of transformation x-gluc substrate was used. From transformed roots at first callus and then plant was regenerated. Finally auxin content from roots and leaves of regenerated plants were measured. Results: Blue color of roots confirmed the successful transformation of samples. Results showed that auxin content in transgenic plants compare to control was increased 80-90 %. Transgenic plants showed shorted internodes and smaller leaf area than non transgenic plants. Conclusion: Regenerated plants from transgenic roots showed higher level of auxin content than non transgenic plants. Change of auxin content and its interaction with gibberellic acid possibly resulted in shorter length of transgenic plants.