Cell and Tissue Journal

Abstract Aim: The purpose of this study is evaluation of seven different varieties of soybean seeds cultivated in Iran based on seed storage protein patterns using SDS-PAGE method.
Material and methods: First, total soluble protein extracted from seeds of seven different varieties of soybean cultivated in Iran including: GK, L17, Sari, 033, 032, Talar and Sahar were measured. The electrophoretic pattern of storage proteins of soybean seeds was evaluated by SDS_PAGE method. Relative density of protein bands was analyzed. Relationships among the seven varieties of soybeans were evaluated based on the presence or absence of protein bands and cluster analysis.
Results: Protein content of soybean seeds showed that total protein in only one variety decreased compared to other varieties. In this study, 11 polymorphic bands on a gel for evaluation of genetic variation were detected and analyzed. Differences in intensities of protein expression were observed among different genotypes. Relationships among the seven varieties of soybeans were studied based on protein patterns and the highest and lowest similarities among varieties were identified.
Conclusion: The protein pattern of soybean seeds based on the presence or absence of protein bands can identify genetic variation and will also introduce specific protein markers that might be used to identify different varieties.
 

Abstract Aim: Electromagnetic field (EMF) is an unavoidable environmental factor for living beings which many investigations have been conducted to evaluate its effect. In this research the effects of EMF on vegetative organs, pollen development, pollen germination and pollen tube growth of Glycine max L. were studied.
Materials and Methods: Exposure to EMF was performed by a locally designed generator which its electrical power was provided by a 220 V and 0.1 A, AC power supply .This system consist of a PVC cylinder  with 20 × 20 cm (diameter and length) and   300 turn coil of copper wire. The structure of vegetative parts and reproductive organs was studied using common methods of cell – histology.
Results: In the stem of treated samples collenchymas layers were increased and formation of xylem tissues was more rapid. In the leaves, spongy parenchyma tissue was deformed and numbers of trichomes were increased, in addition leaves and shoot development were delayed and the size of anthers was also decreased with the deformation of their cell wall. The numbers of pollen and tetraspore, decreased and they were also abnormal in shape. Under EMF treatment, the germination percentage of pollen was decreased and pollen tubes were helicoidal and short.
Conclusions: Low intensity electromagnetic field may have effects on the structure of some organs and developmental characteristic of them in Glycine max L.