Cell and Tissue Journal

Abstract Aim: In this research, the antibacterial effects of essential oil of two medicinal plants, Allium sativum and Rosmarinus officinalis were studied on Staphylococcus agalactiae, S. aureu and Escherichia coli
Material and Methods: Essential oils were prepared from department of medical plants of Arak University. The antibacterial properties of the essenceswere investigated by disk diffusion method in different concentrations of the essences (10, 30 and 50%). MIC and MBC were determined by using different concentrations of essences. Antibacterial activity of Penicillin, Bacitracin and Erythromycin was determined with the discs that were impregnated with antibiotics.
Results: All concentrations of the essential oil (10, 30 and 50%) had antibacterial effect. Antibacterial effects decreased according to reduction of essential oil concentrations. There were no significant difference between Allium sativum and Rosmarinus officinalis in MIC and MBC. There were, however, significant differences among mean zones of inhibition found in essential oils and antibiotics (Penicillin, Bacitracin and Streptomycin) at 10% concentration.
Conclusion: According to antibacterial effect of Allium sativum and Rosmarinus officinalis essential oils on major bacterial mastitis pathogens in cow these plants has the potential to be evaluated as an alternative or adjunct to antibiotics to treat bovine mastitis.

Abstract Aim: The aim of this study was to determining effect of short-term garlic extract supplementation on serum total ‌‌antioxidant capacity(TAC), malondialdehyde and and total peripheral leukocytes counts in non-athlete men after an aerobic exercise.
Material and methods: Twenty non-athlete men (aged 23±3 years, body fat 16±2%, and VO2max 40±3 ml/kg/min) in a randomized and double-blind design were divided in two equal groups: supplement group (garlic extract, 700mg/day) and placebo group (dextrose, 700mg/day). After supplementation period, all subjects were participated in aerobic exercise protocol with75% VO2max on the treadmill for 30 minutes. Primary blood samples in the basic state and before of start supplementation, second blood samples after the end of supplementation period and third blood samples after the exercise were taken. Data were analyzed by repeated measure ANOVA, Bonferroni and independent t test using SPSS at α≤0.05.
Results: Show that short-term garlic extract supplementation had significant effect (p < 0.05) on basal serum TAC. Moreover, MDA, total peripheral leukocytes counts was significantly increased and TAC was significantly decreased (p < 0.05) after 30 min aerobic exercise. The change range in the oxidative and inflammatory indices of placebo group, However, was significantly more than in supplement group (p < 0.05).
Conclusion: Our results suggest that the increase of resting total antioxidative capacity following Garlic short supplementation can cause decrease exercise-induced oxidative damages (Lipid Peroxidation) and inflammatory indices (Leukocytosis) in non-athlete men.