Bioinformatics and Molecular Cytogenetic Study of a Small Region of Chromosome 15 Including Allelic Imbalances in Uterine Cancer Prone Inice
Volume 4, Issue 3, Winter 2013, Pages 297-303
https://doi.org/10.52547/JCT.4.3.297
Abstract Aim: Previous studies using LOH technique on BDII rats showed four distinct Allelic imbalance on chromosome 15. In this research we have studied region 2 including Allelic imbalance by bioinformatic approachs and FISH (Florescence in situ hybridization) technique.
Material and Methods: The confirmed tumors by an expert pathologist were used for cell culture. Metaphase chromosomes were prepared by conventional methods. The corresponding genes labeled probe for hybridization was poured onto slides. The labeled probe was then subjected to detected phase. Slides were studied under a fluorescence microscope using software CW4000 Laica.
Results: Using some of the database we could register 104 genes. But most of them did not have a clear function. According of FISH results, Dlgap5, Fermt2 and Socs4 had amplification and Lgals3 had copy number reduction.
Conclusion: according to our results, the Dlgap5 and Socs4 are probably involve in EAC
FISH study of chromosome 15 in a model effected with endometrial adenocarcinoma cancer
Volume 2, Issue 1, Autumn 2011, Pages 17-24
https://doi.org/10.52547/JCT.2.1.17
Abstract Aim: Main goal of this investigation were verification of gene's behavior which located on RNO15 with allelic imbalance during Endometrium cancer development.
Material and methods: BDII/Han females were crossed to males from two other inbred rat strains known to have low incidence of EAC (BN/Han and SPRD-Cu3/Han). To generate F2 rats the F1 animals were intercrossed. In addition, backcross populations were generated by crossing male F1 rats to female BDII rats. In order to characterize these tumor-specific genetic aberrations in greater detail, we applied molecular cytogenetic methods (FISH, chromosome painting) to 18 rat endometrial adenocarcinoma (EAC) cell cultures.
Results: Analysis in the tumors by FISH analysis showed the presence of small regions of amplifications.
Conclusion: These changes were cryptic so that they occurred inside cytogenetically normal-looking chromosomes. We detected rather specific chromosome aberrations (translocations, deletions, amplifications) often leading to copy number changes in small DNA segments (gains, losses).