Cell and Tissue Journal

Abstract Aim: The aim of the present study was to compare quantitative distribution and histological characteristics of Paneth cells of small intestine in sheep and goats.
Material and Methods: Ten healthy adult sheep and 10 healthy adult goats (5 male and 5 female in both sexes) of the Lori Bakhtyari breed were selected from Shahrekord abattoir. Prepared tissue sections were analyzed at both light and electron microscopic levels.
Results: Paneth cells were distributed in the crypts of Liberkühn, theirs granules were larger and more numerous in deep and cells number showed an increase the proximal of the deudenum towards the distal part of the jujenum followed by a decrease from the proximal of the ileum towards its distal part. In all regions, Paneth cells were more numerous in goats than to sheep. In electron microscopic level, dense granules were found in the apical cytoplasm. Numerical density and surface microvilli of granules were greatest in the jejunum and lowest in duodenum and ileum. In all three regions of jejenum, area of cytoplasmic granules was significantly greater in sheep than in goats.
Conclusion: The result showed that paneth cellular structure is almost similar between two species in light and electron microscope levels.
 
 
 

Abstract Aim: In this study the toxic effect of the three anti hypertensive drugs (atenolol, propranolol and diltiazem) on hepatocytes were investigated.
Materials and Methods: The 8 weeks old adult male NMRI mice were fed with or without the drugs for 1 month. Mice were divided into 4 groups that received atenolol (50 mg/kg/daily), propranolol (80 mg/kg/daily), and diltiazem (180 mg/kg/daily). Food access was stoped 12 to 15 h before the mice were sacrificed and processed for light and transmission electron microscopic evaluation. In addition modified Hepatitis Activity Index was computed too.
Results: In the control group no pathological sign was observed. Main histological changes such as portal infiltration of lymphocytes and piecemeal necrosis in all three experimental groups as well as expanded necrosis in proparanolol group were seen. In addition electron microscopic imaging indicated an increase in the level of free ribosomes and glycogen droplets in the experimental groups. Also in the mice treated with propranolol group mitochondria membrane disruption were seen, whereas in the group treated with diltiazem, the mitochondrial cristae and endoplasmic reticulum Disintegration could be observed.
Conclusion: Chronic oral administration of atenolol, propranolol and diltiazem in male mice caused hepatotoxicity. The most pathological effects were observed in the group treated with diltiazem, though electrone microscopic imaging indicated the propranolol and dilitiazem were more toxic than the other one.