Cell and Tissue Journal

Abstract Aim: The present study aimed to investigate the effects of aqueous and alcoholic extracts of shallot on breast cancer cells compared to carboplatin and taxol treatments. Material and methods: Different concentrations of drugs taxol and carboplatin and aqueous and alcoholic extracts of shallots were prepared. Using Trypan blue and MTT, cytotoxicity on breast cancer cells, in different times and concentrations were evaluated. Utilized Hoechst and propidum iodide staining morphological changes and possible apoptosis of cells were determined. The data statistically analyzed using one-way analysis of variance and the mean level of P Results: The results indicate that shallot alcoholic extract has more inhibitory effects on cancer cells than aqueous extract at concentration range (0.01 to 0.05 g/L). Also, the simultaneous effect of shallot’s extract, accompaniment with carboplatin, caused enhancement in cytotoxicity of carboplatin. On the other hand, accompaniment of aqueous extract with taxol led to reduction of taxol cytotoxicity. Conclusions: This study showed aqueous and alcoholic extracts of shallots has cytotoxicity effects on breast cancer cells on rats affected by breast cancer. This plant is a herb which can be used against breast cancer  can be subjects for further research.

Abstract Aim: In this article, the ability of p < sup>32 radioactive drugs for treatment and control of breast cancer in rat model and also verifying of distribution of drug in rat organs are investigated.
Material and Methods: 10 to 20 mg per kg of DMBA gavaged to SD strain rats. Tumors 15-10 mm in diameter were selected for testing. P32 radioactive drugs injected to developed tumors with interval 3, 5 and 7 days. The distribution of the P32 radioactive drugs in the body of rat were analyzed by NI detector. After the treatment period, animals were anesthetized and the tumor is completely removed under sterile conditions and placed in 10% formalin. Sections of the tumors were obtained and processed using Tissue Processor. After tissue processing and sectioning, the samples were stained with hematoxylin - eosin were examined.
Results: The results obtained from the examining histological sections prepared from tumor tissue confirmed that, the pathology of tumors induced by DMBA in rats used in this study is very similar to human mammary tumors. Five days after injection, we could find tumor's cells and lymph ducts are covered by residual epithelial cells. Many of the cells are separated from the basement membrane and collapsed into the tubular cavity. Rest of the cells has small nuclei, with chromatin condensation mode and cytoplasm was compact and dense. In radius of about 7-8 mm around the site of injection the tumor's cells were necrosis and formed a cavity, inside of tumor.
Conclusions: These observations showed further penetration and influences of radiation emitted from the phosphor 32 in the tumor tissue based on our injection pattern.
Therefore if given the proper distance of injection within the tumor, destroying the tumor's ability to be on time will be shorter.
 

Abstract Aim: The aim of this study was to evaluate the cytotoxic effects of rosemary (Rosmarinus officinalis) extracts on cancerous cells derived from DMBA induced mammary tumor in SD rats.
Materials and methods: Treatment group were gavaged by DMBA (10mg/kg) dissolved in 1ml sesame oil. After tumor development, a part of tissue samples were used for pathological studies using H&E staining to determine the type of the tumor. Cancerous cells were prepared after breaking the tissue using mechanical method. Dried leaves of Rosemary were extracted using digestion method with the help of methanol and filtrated by filter paper. One part of extract was kept away, and to separate the non polar compounds, the rest of the extract was washed with non polar solvents (hexane, dichloromethane, ethyl acetate). Then the solvents were removed by rotary evaporator and then the desirable dilutions of extracts as well as Taxol were prepared. After 24 hours treatment of the cells with the extract and taxol the cell viability was evaluated by Trypan blue dye exclusion and MTT colorimetric assay.
Results: Alcoholic extract and Taxol showed significant cytotoxic effects on cancerous cells but the effect of aqueous extract was weaker.
Conclusion: according to this study alcoholic extract of Rosemary had a dose dependent cytotoxic effect on cancerous cells which was comparable to Taxol cytotoxicity. Moreover these data demonstrated that the induced breast cancer in rat is an appropriate model to simulate human breast cancer.