Cell and Tissue Journal

Abstract Aim: The purpose of this study was to investigate the effects of benfotiamine treatment of hyperglycemic rats on viability of adipose tissue- derived mesenchymal stem cells on sciatic nerve acellular scaffolds.
Material and method: After induction of hyperglycemia (STZ) and treatment with benfotiamine segments for 4 and 8 weeks from the middle part of the sciatic nerves were  decellularized using Sandell method and seeded with  mesenchymal stem cells derived from adipose tissue. after 8 days of culture cells viability in the culture medium was evaluated by MTT assay and  adhesion of the cells to the scaffold was examined by scanning electron microscopy (SEM).
Results: In comparison with intact group, cell viability of untreated hyperglycemic rats was significantly decreased while, there was no significant difference between benfotiamine  treated group and intact. Results of electron microscopy confirmed adhesion of  the cells on the scaffolds.
Conclusion: In hyperglycemic condition it is likely that glycosylation of ECM constituents and the production of AGEs decrease the inducible effects of ECM on the level of cell viability and probably cell adhesion to the scaffolds. It seems that benfotiamine treatment of hyperglycemic rats by reduction of glycation and AGEs production may prevent the structural changes of the ECM.
 

Abstract Aim: The main goal of this research was to prepare a three-dimensional matrix from gingival palate tissues and investigate the possible application of this scaffold ​​in cell culture and tissue engineering.
Materials and methods: In order to fabricate the scaffolds, the biopsy samples of human palate gingival tissue were preparated surgically and divided in 5 groups then decellulization of the samples were carried out via physical method (put in nitrogen tanks and rinsing with distilled water) as well as chemical method using different concentrations of SDS (0.1%, 0.25%, 0.5%, 0.75% and 1%). Furthermore to evaluate the scaffold prepared with 1% SDS, embryonic like cells from blastema tissue were seeded on the three-dimensional scaffold.
Results: Concentration of SDS below 0.5% caused significant reduction (p < 0.05) of decellulization of the tissues. Microscopic studies of blastema tissue on the scaffold in different days revealed the penetration, migration, adhesion and differentiation of the cells.
Conclusion: This study showed that, it is possible to prepare a natural scaffold frome palatal gingiva tissue using SDS treatment. On the other hand, the results of histologic studies showed that the decellulized scaffolds of palatal gingival might be suitable as three-dimensional bioscaffold for movement, adhesion, differentiation and migration of cells. More investigation is needed to determine the identity of the differentiated cells which further it can help to improve our knowledge about cell-matrix interaction.
 

Abstract Aim: Extracellular matrix (ECM), in addition to physical role can control the cellular behavior such as proliferation, differentiation and migration.  With respect to dimension, architecture, cell polarity and microenvironment similar to in vivo, it is important to study cellular behavior in the three dimensional culture compare to two dimensions. In this study, ECM derived from bovine articular cartilage and cancellous bone were used as a three dimensional environments to study the movement and polarity of cells from blastema tissues.
Material and Methods: In order to remove cells from the cancellous bone and articular cartilage, physicochemical methods including  snap freeze–thaw and  sodium dodecyl sulfate (SDS) as an ionic detergent were used.  Then the prepared  decellulized matrix  were assembled with  the rings of the blastema  tissues originated from pinnas of male New Zealand white rabbits and cultured in different days in vitro.  
Results:  The removals of the cells have been confirmed by histotechniques. In addition adhesion, polarity and migration of the blastema cells around the trabeculae of bone and articular cartilage ECM took place.
Conclusion: This study showed that the co-culture of blastema tissue with dynamic cells and 3D scaffolds might be a suitable model to study cell behavior such as migration and polarity in vitro.