Cell and Tissue Journal

Abstract Aim: The purpose of this study is evaluation of seven different varieties of soybean seeds cultivated in Iran based on seed storage protein patterns using SDS-PAGE method.
Material and methods: First, total soluble protein extracted from seeds of seven different varieties of soybean cultivated in Iran including: GK, L17, Sari, 033, 032, Talar and Sahar were measured. The electrophoretic pattern of storage proteins of soybean seeds was evaluated by SDS_PAGE method. Relative density of protein bands was analyzed. Relationships among the seven varieties of soybeans were evaluated based on the presence or absence of protein bands and cluster analysis.
Results: Protein content of soybean seeds showed that total protein in only one variety decreased compared to other varieties. In this study, 11 polymorphic bands on a gel for evaluation of genetic variation were detected and analyzed. Differences in intensities of protein expression were observed among different genotypes. Relationships among the seven varieties of soybeans were studied based on protein patterns and the highest and lowest similarities among varieties were identified.
Conclusion: The protein pattern of soybean seeds based on the presence or absence of protein bands can identify genetic variation and will also introduce specific protein markers that might be used to identify different varieties.
 

Abstract Aim: Regarding economic, medicinal, food and decorative importance of Artemisia genus,this study was carried out to determination of intraspecific diversity in the Artemisia incana populations collected from East Azerbaijan. 
Materials and methods: To study of intraspecific diversity in A. incana in East-Azerbaijan D.S.S. method was used. Six special habitats of this population were recognized. Seeds of populations as well asand floristic-ecologic data were collected from each special habitat. Seed storage proteins were subjected for electrophoresis studies.
Results:  In survey of all special habitats, 54 species were distinguished as associated species. Analysis of floristic data (Floristic compositions of each special habitat as floristic marker) led to identification of 4 separate groups that was indicated the existence of intraspecific diversity in this species. Analysis of ecologic data was also confirmed the groupment. The analysis of seed storage protein electrophoresis banding pattern showed three quite obvious groups.
Conclusion: In the survey of electrophoresis pattern, existence of differences regarding number and density of the protein bands indicating existence of intraspecific diversity in the populations of           A. incana. Therefore in this species, the grouping that introduced with floristic marker, confirmed also with ecological and electrophoresis markers. This means that floristic grouping can only be used as a cost-effective and efficient method for studying of intraspecific diversity.