Enhanced Production of human Growth Hormone in CHO Cells through the Control of Culture Conditions
Volume 6, Issue 1, Spring 2015, Pages 97-106
https://doi.org/10.52547/JCT.6.1.97
ZS A, SH Z
Abstract Aim: The purpose of this study was enhancement of hGH synthesis by chemical components addition to Serum-Free CHO cells culture and investigate the characteristics of produced hGH.
Material and Method: The effects of different treatments including various concentrations of glycerol, DMSO, NaBu and ZnSO4 in CHO cells were investigated and the GH production was assessed using ELISA method. Cells are seeded into each well of a 12 well plate containing DMEM-F12 and 10% FBS. After 24 hours, media was aspirated and replaced with SFM and treatment with different concentrations of chemicals and rhGH production was assessed using ELISA, dot blotting and western blotting.
Results: Concentrations of 1%DMSO, 0.5% Glycerol, 1mM NaBu and 50 μM ZnSO4 were caused to elevate the expression of rhGH in the CHO cells.
Conclusion: These findings indicate that control of culture aid to development of an efficient large-scale and industrial process for the production of rhGH.