Cell and Tissue Journal

Abstract Aim: In this study, the extract of earthworm (Eisenia foetida) (in solvent: olive oil and vaselline) was applied as a novel healing stimulator on suture wound in goldfish (Carassius auratus).  
Material and Methods: Groups were: negative control (Intact skin), positive control (suture wound with phenytoin), pseudo-control (sham: suture wound with solvent), and experimental (suture wound with 10 mg earthworm extract). A 2 cm longitudinal cut was made from head to tail, and then suture was applied followed by daily treatments. After 14 days, blood samples were collected. Macroscopic and microscopic observations were also done. Meanwhile, the content of lipid peroxidation (LPO/MDA) was measured in fish blood sera.
Results: surface and microscopic observations of wounds revealed a better result for earthworm extract treatment in suture wound healing when compared with phenytoin. MDA level in goldfish blood sera was lesser in earthworm extract-treated groups compared to other groups.
Conclusion: Earthworm extract is a new candidate in aquatic wound healing. Some factors of the extract may enter the blood and ultimately activate the antioxidant systems leading to decrease the content of LPO in fish blood sera.   
 

Abstract Aim: This study is examination skin wound healing after using of lotus corniculatus L. aerial part extract in comparison with Phenytoin ointment in rat.
Materials and methods: After anesthesia of 80 Wistar male rats, a rectangular wound was produced at back each of which. Healing woud steps was studied in 8 treated groups: control (A), 1% Phenytoin cream (B), 10% extract ointment (C), 20% extract ointment (D), 40% extract ointment (E), 1% Phenytoin cream + 10% extract ointment (F), 1% Phenytoin cream +20% extract ointment (G), 1% Phenytoin + 40% extract ointment (H). Medication and measuring wounds were done using digital images analysis for 21 days. Samples were collected from wound healing sites for microscopy after 3, 7, 14 and 21 days. Wound healing was determined for all of treated groups. Data were analyzed using SPSS18.
Results: Healed wound area mean in E and H groups was lower than comparing to other groups (p < 0.01). Also based on histopathological results, E and H groups showed more healing wound in comparison with other groups.
Conclusion: According to anti-inflammation, anti-microbial and healing effects of Lotus corniculatus in comparison with 1% Phenytoin, it is believed that the plant compounds are more effective in full thick skin wound healing.