Sequencing of LEAFY homologous gene in Solanum villosum Mill.
Volume 6, Issue 1, Spring 2015, Pages 51-58
https://doi.org/10.52547/JCT.6.1.51
M A, F R, Gh Sh, H S
Abstract Aim: The purpose of this study was to identify LEAFY homologous genes in a type of nightshade (Solanum villosum Mill.).
Material and Methods: Total RNA was isolated from shoot apical meristem tissue of S. villosum and was used for First-strand cDNA synthesis. The specific primers were designed based on nucleotide sequence alignment of LEAFY homologous genes from other plants and were used in RT-PCR. The RT-PCR products were purified and sequenced.
Results: The results indicated amplification of 540 nucleotides fragment, and named SvLFY. Comparison of SvLFY deduced protein sequences with LEAFY homologous proteins from other species showed high similarity between this fragment and in other C-terminal regions.
Conclusion: These results suggest that SvLFY may play a similar role as LEAFY in flower development and could act as a flower meristem identity gene in Solanum villosum Mill.