Cell and Tissue Journal

Abstract Aim: The aim of this project is finding a relationship between body characters of Hirudo orientalis with testis sac and spermatic follicles structure in order to find a relationship between these characters and sexual maturity, regarding farming goals.
Material and methods: 30 individuals of H. orientalis were collected From 3 ponds located in Gonbad-é-Kavous city in May, July and November. Body length, width and weight, Testis sac diameter, testis sac diameter to body diameter ratio and spermatic follicle numbers obtained for each sample. For understanding starvation effect on testis sac, 20 individuals of collected leeches were starved for 120 days. Data were analyzed using ANOVA test and SPSS 16 software.
Results: Body length, width and weight, testis sac diameter, testis sac diameter to body diameter ratio and spermatic follicle numbers showed significantly reduction in May comparing with July and November (p < /em><0.05). Mature spermatic follicles were observed in samples with more than 3 gr weight, >10.3 cm length and >0.79 cm diameter in July and November. All of studied characters were reduced with the exception of body length and testis sac diameter to body diameter ratio in starved samples (p < /em><0.05).
Conclusion: This study showed that testis sac structure and spermatic follicle numbers can directly related to body weight and diameter in H. orientalis but not to body length.

Abstract Aim: Lizards are one of the most diverse and successful vertebrates in hot deserts of the world that biologists focus on their reproduction and gametogenesis.
Materials and Methods: This research is experimental study and the aim of that is to investigation of spermatogenesis steps and sperm production limit identification belongs spring three months. 10 male lizards (Laudakia Caucasia, Syn: Stellio caucasicus: Agamidae) were collected at the end of each month. After determination and morphological studies, their testes were dissected of animal body and prepared for histological studies using light microscopy. Quantitative data were analyzed using SPSS by ANOVA and Kruskal-Wallis tests (p < /em><0.05).
Results: Results showed that the spermatogenesis is started in the lizard from beginnings April and is gradually activated more with warming weather, As maximum spermatogenesis and mature spermatozoa production were observed in the end of June.
Conclusion: Based on histological evidences, spermatogenesis step is activated from early spring and is continued to the late of spring. Maximum sperm production is in June.