Cell and Tissue Journal

Abstract Aim: The aim of this study was to investigate the preventive role of vitaminE (Vit.E) on spermatogenesis indexes in rats following exposure to Bisphenol A. Materialand and Methods: Adult male wistar rats with the mean body weight of 231±10g were randomly divided into 4 groups (n=6): control, BPA (250mg/kg/day), Vit.E (150mg/kg/day) and BPA + Vit.E.  Oral treatment was performed three times a week till 56 days. At the end of the treatment, the rats were killed and their left testis were weighed, fixed and stained using Heidenhain's Azan  methed. Histological and morphometrical analysis of spermatogenesis was carried out. Data were analyzed using One Way ANOVA and  the means were considered significantly different  at p < strong>Results: Testis weight (p < 0.01) significantly decreased in the BPA group compared to the control. A significant decrease (p < 0.001) in the mean diameter of seminiferous tubules and the germinal epithelium thickness and the indexes of tubular differentiation, spermiogenesis and meiosis were also observed in the testicular tissue of rats treated with BPA compared to the control ones. In the BPA +Vit.E group, the mentioned parameters increased significantly to the control level. Conclusion: Vitamin E can compensate for the undesired effects of BPA on spermatogenesis and therefore could be considered as a therapeutic supplement in the case of BPA toxicity.  

Abstract Aim: Infertility is the failure of a couple to engender after endeavoring at least one year of unprotected intercourse. male factor infertility accounts for approximately 50 percent of causes. Tumor necrosis factor-α (TNFα) is a multifunctional cytokine. TNF-α plays important role in the regulation of cellular processes related to spermatogenesis. There are two variants of the cell receptors that interacts with TNF-α. In the present study, the association of TNFR1 36A/G polymorphisms with idiopathic male infertility in the Guilan population was studied. Materials and Methods: This study consists of 106 infertile men and 114 fertile men as control group. Blood samples were taken and genomic DNA was extracted. Then genotypes and allele frequencies were assessed by PCR-RFLP method and the statistical analysis was performed by MedCalc software. Results: The frequencies of AA, AG, GG genotypes in patients were 41.5%, 7.5% and 50.9%, respectively and in controls were 45.6%, 33.3%, and 21.1%, respectively. The frequencies of A and G in patients were 0.45 and 0.55, respectively and in controls were 0.62 and 0.38, respectively. The results showed that there is a significant association between genotype frequency (p= 0.002) and allele frequency (p= 0.0004) in infertile and control groups. Conclusion: In conclusion, the subjects with G allele and GG genotype appears to be at greater risk of developing idiopathic infertility in Guilan province. Further studies with larger numbers of patients are required to elucidate the potential role of TNFR1 polymorphism in male infertility.

Abstract Aim: To investigate spermatogenesis in the Marsh frog living in Makhmalkooh, Khorramabad, and determine which type of spermatogenesis found in this species.
Material and Methods: 34 male frogs were collected from the study area during September to February. First, the specimens were identified by measuring biometric, meristic characters and according the present keys. Then, abdominal cavity was dissected and the gonads were out and processed to prepare for histological sections methods. The tissue sections were finally examined for histological features and three quantitative parameters.
Results: Microscopic observations showed that in this species the event of sperm releasing starts in March and continue to June, during which spermatogenic cysts are observed on the wall of seminiferous tubules. This event ceases during July to September when spermatogenesis enters to its resting phase. During the resting phase the cysts are not found in the seminiferous tubules. A regenerative phase was distinguished during September to March as the number of cell increased in germinal layer. Quantitative surveys also illustrated that during March to September, diameter of the tubules and their germinal layer initially increased and then declined while the lumen’s diameter showed an opposite flux when compared with two other parameters.
Conclusion: Based on the results presented here, it seems that in this species the spermatogenesis does not occur as continuous but it is carried out in the form of semi-continuous or potentially continuous.
 

Abstract Aim: Lizards are one of the most diverse and successful vertebrates in hot deserts of the world that biologists focus on their reproduction and gametogenesis.
Materials and Methods: This research is experimental study and the aim of that is to investigation of spermatogenesis steps and sperm production limit identification belongs spring three months. 10 male lizards (Laudakia Caucasia, Syn: Stellio caucasicus: Agamidae) were collected at the end of each month. After determination and morphological studies, their testes were dissected of animal body and prepared for histological studies using light microscopy. Quantitative data were analyzed using SPSS by ANOVA and Kruskal-Wallis tests (p < /em><0.05).
Results: Results showed that the spermatogenesis is started in the lizard from beginnings April and is gradually activated more with warming weather, As maximum spermatogenesis and mature spermatozoa production were observed in the end of June.
Conclusion: Based on histological evidences, spermatogenesis step is activated from early spring and is continued to the late of spring. Maximum sperm production is in June.
 

Abstract Aim: Electro magnetic fields (EMFs) on spermatogenesis, blood serum protein level and alkalin phosphatase of mice have been investigated.
Material and methods: Male adult Balb/c were arranged in 3 groups (control, sham-exposed, experimental-n=6-). In experimental group, mice received EMFs(120 gauss and frequency of 100 Hz) twice every time 7 hours in two consecutive days. After 48h, the animals were   be weighed and dissected. The number of germ cells in testes with histotechnic assay, assessment of alkalin phosphatase with auto analyzer and measurement of blood serum protein with electrophoresis assay were investigated.
Results: No significant difference observed in mice weight and testes weight. The number of spermatogonia and secondry spermatocyte showed significant increase (p≤0.05)  in  experimental group rather control, sham-exposed. Assessment of serum proteins indicated between 5 essential proteins in blood, percentage of alpha-globulins showed significant reduction (p≤0.05) in experimental group. Evaluation of alkalin phosphatase didn’t show significant difference.
Conclusion: Exposing Balb/c mice to EMFs with low frequency (120 gauss and frequency of 100 Hz) has led to changes in testis tissue such as increasing secondry spermatocyte, syncitium state in spermatogenic cells, distruction interstitial tissue.These changes can cause reduction of fertility.