Rubinia pseudoacacia – Rhizobium Symbiosis for Phytoremediation of Petroleum Polluted Soil
Volume 4, Issue 1, Summer 2013, Pages 1-12
https://doi.org/10.52547/JCT.4.1.1
Abstract Aim: Plant-microbe interactions are considered to be important processes determining the efficiency of phytoremediation of petroleum pollution. The use of legume-Rhizobium is a suitable method for modification of petroleum-contaminated soil. The aim of this study was to evaluate the phytoremediation potential of Acacia– Rhizobium symbiosis in petroleum-polluted soil. Material and methods: The three-day acacia-seedlings were transferred to hydroponic medium and inoculated with rhizobium. Then, the 13-days seedlings transferred to polluted soil with different concentration of crude oil, that is; 0% as control and 1-5% (vol. /Wight), at the start and end of the 90-days period. Soil samples were analyzed for hydrocarbon removal (total hydrocarbon, n-tridecan, n-tetradecan and n-pantadecan) by GC-FID. Contents of Pb, Zn and Cd were measured with atomic absorption from soil and plant roots. The data were statistically analyzed with the help of SPSS11 and Duncan ‘test. Results: The results showed accumulation of heavy metal in plant roots and it’s reducing in soil. Hydrocarbon reduction was found over the course of the experiment in all treatments. The maximum removal was obtained in plants inoculated with rhizobium at 4% treatment, in which inoculated-acacia removed 97-100% of the hydrocarbons from soil. There fore inoculation Rubinia pseudoacacia with Rhizobia are effective in removing TPHs and heavy metals from petroleum polluted soil. Conclusion: Based upon these results, Rubinia pseudoacacia L. inoculated with rhizobium can be used as lead and cadmium bioaccumulator in petroleum pollution and was selected for the phytoremediation of petroleum-contaminated soil.
Evaluation of the Phytoremediation of Robinia pseudoacacia L. in Petroleum- contaminated Soils with Emphasis on the Some Heavy Metals
Volume 2, Issue 4, Winter 2012, Pages 437-442
https://doi.org/10.52547/JCT.2.4.437
Abstract Aim: Petroleum is a complex mixture of thousands of hydrocarbon and non-hydrocarbon compounds, including heavy metals which potentially are carcinogenic and mutagenic. Phytoremediation has been shown to be effective for degradation or removing petroleum contaminants. Selection of plant species for phytoremediation, however, is complicated.
Material and Methods: The effects of petroleum pollution of soil (0%, 1%, 2%, 3% and 4% V/W) on the proline, total protein , lead, cadmium and zinc contents in Acacia leaveswere investigated using methods of Bathes, Bradford and Atomic Absorption, respectively. The data were statistically analyzed using SPSS v11 and Duncan’s test.
Results: The results showed that the total protein and proline contents increased significantly (p≤0.05) as the level of pollution increased. The highest proline content was measured at 4% treated plants. Proline accumulation is a common physiological response in many plants against a wide range of biotic and abiotic stresses. The results showed that lead and zinc accumulated by leaves. The leaf lead values were markedly, 20.8-fold, enhanced in 1%. There was not significant differences among cadmium levels in treatments and the control plants.
Conclusion: Based upon these results, Robinia pseudoacacia L. can be used as bioaccumulator in petroleum pollution and were selected for the further investigation of the phytoremediation of pb-contaminated soil.