Effect of H2O2 and Melatonin on In vitro Oocyte Maturation
Volume 2, Issue 4, Winter 2012, Pages 387-393
https://doi.org/10.52547/JCT.2.4.387
Abstract Aim: The aim of this study was to determine the effect of stress oxidative (H2O2) and different concentrations of melatonin on nuclear maturation of immature oocytes in sheep.
Material and Methods: Ovary collection and oocyte recovery was carried out by standard method. Oocytes culture was in the following conditions: A: TCM199+10% FBS, 5µg/ml FSH, 0.01IU/ml LH, 100 IU/ml penicillin and 100 IU/ml streptomycin, B: A + H2O2 (300 µM) at 38/5 C˚, C: B + 1 µM melatonin and D: B + 10µM melatonin.
Results: This study showed that H2O2 significantly (p < 0.05) decreases nuclear maturation in compare to control (14.7 vs. 84. 9). 0, 1 and 10 µM melatonin could improve oocytes to reach to metaphase-II stage (respectively 14.7 vs. 43.29, 54.12). But increasing melatonin dose from 1 to 10 µM, did not have any significant effect on oocytes maturation.
Conclusion: The results of this study showed that melatonin improves sheep oocytes maturation during oxidative stress.