Comparison of sperm parameters and its functional characteristics between fertile and infertile men with varicocele
Volume 6, Issue 4, Winter 2016, Pages 523-532
https://doi.org/10.52547/JCT.6.4.523
F B, M T, L A, MH N
Abstract Aim: The aim of this study is to compare sperm parameters (concentration, motility and morphology), DNA damage, percentage and intensity of ROS (Reactive Oxygen Species) and relationship between these parameters in the fertile and infertile men with varicocele.
Material and Methods: In this study, the semen samples from 83 individuals with varicocele and 32 fertile men were studied. Sperm parameters according WHO (World Health Organization) guidelines, protamine deficiency (chromomycin A3 staining), sperm DNA damage (TUNEL assay), percentage and intensity of ROS (DCFH-DA staining) were assessed in fertile and infertile men with varicocele.
Results: The results of this study showed that sperm concentration, percentage of motility and normal morphology were significantly lower in individuals with varicocele compared to fertile individuals. In addition, percentage of sperm DNA damage, protamine deficiency and ROS positive were significantly higher in infertile men with varicocele.
Conclusion: The increase of stress oxidative and testicular temperature in infertile men with varicocele, lead to decrease the quality of sperm parameters as well as negative effects on sperm chromatin integrity. Therefore the process of spermatogenesis and the rate of fertility were affected in these individuals.
Effect of H2O2 and Melatonin on In vitro Oocyte Maturation
Volume 2, Issue 4, Winter 2012, Pages 387-393
https://doi.org/10.52547/JCT.2.4.387
Abstract Aim: The aim of this study was to determine the effect of stress oxidative (H2O2) and different concentrations of melatonin on nuclear maturation of immature oocytes in sheep.
Material and Methods: Ovary collection and oocyte recovery was carried out by standard method. Oocytes culture was in the following conditions: A: TCM199+10% FBS, 5µg/ml FSH, 0.01IU/ml LH, 100 IU/ml penicillin and 100 IU/ml streptomycin, B: A + H2O2 (300 µM) at 38/5 C˚, C: B + 1 µM melatonin and D: B + 10µM melatonin.
Results: This study showed that H2O2 significantly (p < 0.05) decreases nuclear maturation in compare to control (14.7 vs. 84. 9). 0, 1 and 10 µM melatonin could improve oocytes to reach to metaphase-II stage (respectively 14.7 vs. 43.29, 54.12). But increasing melatonin dose from 1 to 10 µM, did not have any significant effect on oocytes maturation.
Conclusion: The results of this study showed that melatonin improves sheep oocytes maturation during oxidative stress.