Cell and Tissue Journal

Abstract Aim: The aim of this study was to evaluate histometrical and stereological properties of the different tissue layers in male and female goats.
Material and Methods: To study the microscopic structure of the goat esophagus by stereological method, the eight healthy adult goats from both sexes were selected and the cervical region was dissected and esophagus removed. The obtained samples were fixed in 10% buffered formalin solution and then prepared tissue sections, stained with hematoxylin and eosin and studied using light microscopy.
Results: Results showed that there are significant variation in micrometry thicknesses of the submucosal and muscular layer at the cervical and thoracic regions (p < /em><0.05). The stereological results showed that mean volume of the esophageal muscular layer was significantly greater in male than female, but in other layers significant differences between both sexes was not observed (p < /em>>0.05).
Conclusion: Although morphometrical results showed that there are significant variations in different layers of the esophagus tissue, but stereological results showed no significant difference.
 

Abstract Aim: As there was no studies available regarding the morphological structure of skin of (Barbus grypus) therefore this research work was performed to help the other studies such as histopathology of the fish.
Material and Methods: Skin of the different part of the head of 20 Barbus grypus with 5mm thickness were removed and fixed in bouin solution. The routine procedures for preparation of tissues were followed. For transmission electron microscopic study, the samples after primary and post-fixation were dehydrated and embedded in resin. Then, thin sections 50 µm were prepared and stained with uranyl acetate.
Results: according to histomorphological examination, skin of head, lip and barbel was made of epidermis and dermis which were located on a loose connective tissue called hypoderm. In histometric study, skin of studied area, was divided into different regions in (Barbus grypus)based on cells distribution. In the micrograph of TEM, goblet cells containing many mucus granule, spinous and club cells with hyper chromatin nucleus were observed, which presence of hyper chromatin nucleus was more obvious in the club cells. 
Conclusion: The light and TEM studies on the skin in studied region of (Barbus grypus), showed there was differences and some similarity with the other reported species.