Cell and Tissue Journal

Abstract Aim: In this study, the genotoxic effect of low frequency electromagnetic wave  that is similar to mobile phones in wavelength, as a main source of producing this waves, in combination with a known aneugen (vinblastine) was investigated on the cultured cells.
Material and Methods: L929 cells were exposed to two forms of continues and discontinues electromagnetic waves with wave length of 900MGh in absence and presence of 1.5 ng/ml of vinblastine for 2 h. Investigation of induced chromosomal abnormalities and cell viability in all treated groups were performed by micronucleus assay on binucleated cells and MTT assay, respectively.
Results: The frequency of micronuclei of cells in continuous and discontinuous electromagnetic fields was significantly higher in comparison with control and cells in turned off electromagnetic field, which represented higher chromosomal aberrations. Co-treatment of the cells with vinblastine did not increase this frequency. Analysis of toxicity showed no decrease in cell viability in all treated groups.
Conclusion: findings represented the ability of electromagnetic field, especially in form of discontinues, in inducing chromosomal abnormality. Also, co-treatment of cells with electromagnetic filed and vinblastine did not elevate the level of chromosome abnormality.
 

Abstract Aim: In the present study, the antioxidant effect of vitamin C on decreasing the induced chromosomal damages by low-frequency electromagnetic field on bone marrow erythrocytes of male Balb/C mouse has been investigated.
Material and Methods: In this experimental study, 48 adult male mouse (Balb/C) were randomly divided into four equal groups: control, test 1, test 2 and test 3. Control group were kept in normal conditions and the mice in test1 and test3 groups were intraperitoneally injected with 50 mg / kg of vitamin C for 8 consecutive days. Then the mice of test 3 and test 2 groups after the fifth day were exposed to electromagnetic waves with frequency of 50 Hz and intensity of 50 Gauss for 4 days and 4 hours per day. The mice in all different groups were sacrificed and micronucleus test were performed on the bone marrow polychromatic erythrocytes. The data were analyzed using spss software and ANOVA test at level p < 0.05.
Results: The frequency of micronucleus in bone marrow polychromatic erythrocytes of test3 group (3.107± 0.276) in comparison with test 2 group (9.29 ± 0.881) showed a significant reduction             (p < 0.001) .
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Conclusion: Vitamin C caused significant decrease in induced chromosomal damages by low-frequency electromagnetic waves in bone marrow polychromatic erythrocytes of adult male Balb/C mouse.