Cell and Tissue Journal

Abstract

Abstract Aim: Electromagnetic field as a physical stimulus, willingly or unwillingly are affected cellular processes. In this study morphology changing and proliferation rate of mesenchymal stem cells were investigated in presence of electromagnetic field (EMF) and nitric oxide (NO). Material and methods: The stromal stem cells were isolated from the Rat bone marrow and incubated. After several passages of the harvested cells, Deta-NO as a donor of nitric oxide was then added to cell culture. These cells were also treated by EMF (50 Hz and 20 mT). The MTT test was used for estimating proliferation rate of the cells. To estimate the proportion of the cell line in different phases of cell cycle due to treatment with NO and EMF, cellular DNA contents were measured by flow cytometry. Result: The results demonstrated decreasing proliferation rate of the stem cells exposed with EMF and NO compared with the control group. We found that the rate of decrease is highly related to the concentration of NO. The double treatment of EMF and nitric oxide was yielded to an obvious arrest in the cell cycle at G2/M phase. Nitric oxide associated with EMF also changed the cell morphology and increased the cell motilities. Conclusion: The changing of cell morphology and reduction of proliferation rate of the stem cell can be considered as a symptom showing the beginning of cell differentiation.

Abstract Aim: The aim of the present study was to evaluate the effects of different concentrations of Chir99021 on ovine fetal marrow-derived mesenchymal stem cells (BM-MSCs) expansion in culture.  Material and Methods: BM-MSCs were isolated from ovine fetal and cultured. Passaged-3 cells were examined for their differentiation potential into osteocytes and adipocytes. In the present study, BM-MSCs from ovine fetal were plated in the presence of 0, 0.5, 1, 1.5, 3 and 5 μM of Chir9902. During the cultivation period, the cultures were statistically compared in terms of induces of cell growth including the number of colonies, population doubling number (PDN), doubling time (DT) and the number of viable cells. Furthermore, expression of the beta-catenin was evaluated. The culture without Chir99021 was taken as the control group.  Results: Our findings indicated that, addition of 0.5 and 1 µM of Chir99021 to medium significantly improved overall proliferation compared to that of control group as well as 5µM of Chir99021(p < 0.05). Furthermore, Five days after treatment with small molecule showed that the treatments with 0.5 and 1 µM Chir99021 formed higher Colony Forming Unit-Fibroblast (CFU-F) compared to control and 5 µM of Chir99021. The expression of beta-catenin was significantly higher in culture supplemented with 1 μM of Chir99021 compared to that in groups containing 0.5 and 1.5 μM (p < 0.05). Conclusion: In conclusion, using Chir99021 at concentration of 1 μM could enhance in vitro proliferation of ovine fetal BM-MSC; however administration of high concentration of this small molecule had toxic effects on these cells proliferation.

Abstract Aim: In this study, it was done to evaluate the efficiency of both PRP and Fibrin Glue scaffolds in producing suitable environment for the growth of mesenchymal stem cells.
Material and Methods: In this study, the preparation of PRP and Fibrin Glue Scaffold were carry out and Mesenchymal Stem Cells (MSCs) isolated from adipose tissue. The mesenchymal phenotype of these cells was determined by mesenchymal surface marker using flow cytometry. Then, MSCs were cultured, and on the third passage (P3)stage, they were seeded separately on the two scaffolds and after 48 hours of cell culture, the ability of the scaffolds seeded cells was evaluated by MTT assay for cells viability.
Results:Flow cytometry results showed that human adipose-derived mesenchymal stem cell expressed CD44, CD90 and CD105 surface markers. Also, the results of this study showed that the active PRP could be creating a more suitable environment for the survival and proliferation of mesenchymal stem cells in compare with Fibrin glue
Conclusion:It is suggested that the PRP as a protective scaffold could be used for mesenchymal stem cells growth to provide effective strategies in order to tissue engineering development and regenerative medicine.

Abstract Aim: The main goal of this research was to prepare three-dimensional matrix of gingival palate tissues and compare the behavior of bone marrow mesenchymal stem cells and blastema on the scaffolds.
Material and methods: The tissues obtained from gingival surgeries
in periodontal clinic were decellularized using detergents including sodium dodecyl sulfate and Triton X-100 then after washing and sterilization, were used as scaffold for culturing the rat’s mesenchymal stem cells. The scaffolds were studied by light and electron microscopy before and after 1, 2, and 4 weeks of culture. In addition the prepared scaffolds were assembled within the rings of blastema tissues from rabbit^,s pinna. Then the scaffolds were similarly studied by histological techniques after 1, 2, and 4 weeks of culture.
Results: Scanning electron microscopy showed that epithelial matrix and collagen fibers in connective tissue remained intact. In both samples epithelium-like structures were observed. Also in the blastema cells migrating to the scaffolds cell secretion was also observed.
Conclusion: Human gingival matrix can be a suitable scaffold for studying the cell behaviors. More research should be carried out to determine the identity of differentiated cells, which finally can improve our knowledge regarding the cell-matrix interactions.