Cell and Tissue Journal

Abstract

Abstract

Abstract Aim: In the present study the effect of oxidative stress resulted from hydrogen peroxide on some secondary metabolites and changes on intra cellular hydrogen peroxide has been explored. Material and methods: To induction of callus Periwinkle leaf was sterilized and put on MS medium. Calluses were treated by different concentrations (1, 5 and 10 µM) of hydrogen peroxide for 6 days. Total alkaloid, phenolic components and felavonoids as well as antioxidant potential on the basis of Iron reduction were determined.  Data were analyzed using one way ANOVA. Results: Addition of hydrogen peroxide resulted in significant (P≤0.05) increase of intra cellular hydrogen peroxide compared to the control. Total alkaloids, flavonoids and phenols content showed that hydrogen peroxide caused a significant increase (P Conclusion: Hydrogen peroxide increased intra cellular levels of hydrogen peroxide. Secondary metabolites such as alkaloids and phenolic compounds and total flavonoid were boosted as well. Therefore tensions may be applied to increase them.

Abstract Aim: Reduction in ozone layer diameter as a result of increasing amount of pollutant causes veduced absorbance of sun light UV rays by this layer. Consequently, living organisms, such as plants are damaged. Occurring photochemical changes in plants are some adaptation, defense and confronting reactions against these damages. So, studies of these changes are important in higher plants.
Material and methods: Studing ten cultivation groups seed and field plants consist of control and UV-C treated (totally 1-20 hours) of Coronilla varia L. were prepared in equal soil and cultivation condition. All of ninety-day old plants were used for chlorophyll-metry and flavonoid studies using 2-dimentional paper and thin layer chromatography methods.
Results: Chlorophyllmetric results showed a decrease in chlorophyll content in UV-C treated plants in comparison with the control. Comparising the leaf flavonoids showed that the number and kind of leaf flavonoid changes in UV-C treated plants in comparison with the control. These changes include Loteulin and Vitexin in treated plants and absence of them in control, Kaempferol presence in the treated field plants and absence of them in the others, Chrysin in the control and absence of them in the treated plants and the disappearing of Apeginin, Isorhamnetin, Rhamnetin, Narengenin, Myricetin and Quercetin in treated field samples.
Conclusion: It is believed that phytochemical changes consisting of flavonoids kind and number varieties are defensive reactions of plant against physiological stresses such as UV-C.
 

Abstract Aim: In this study, the effects of Ni and Zn to leaf anatomy, some flavonoids and nitrotoxin of Coronilla varia were investigated.
Material and Methods: 40 days aged grown Coronilla varia L. plants in equal growth condition were treated with different concentration of ZnCl2 (0, 7.5, 15, 22.5 and 30 mM) and NiCl2 (0, 2.5, 5, 7.5 and 10 mM) for 24 and 72 hours in hydroponic culture and the effects of imposing heavy metals on Leaf anatomy, some flavonoids of control and treated plants examined and compared using 2-DPC (Two Dimensional Paper Chromatography) and TLC (Thin Layer Chromatography) methods. Nitrotoxin measured using spectrophotometer too. All of Data compared together.
 Results: Results showed anatomical changes in polluted plants responses to Zn and Ni. In general results showed a wide changes in leaf flavonoids profile with increases number of total flavonoids at Zn and Ni stress. However, Nitrotoxin increased significantly when Zn and Ni increased.
Conclusion:  Probably, C. varia L. can tolerate against heavy metals stress of Zn and Ni with changes of flavonoids content and nitrotoxin.