The effect of IAA on stress resistance of tobacco seedlings (Nicotiana plumbaginifolia) under in vitro culture conditions
Volume 9, Issue 1, Summer 2018, Pages 86-101
https://doi.org/10.52547/JCT.9.1.86
Abstract Aim: In this study, the effect of IAA on salt tolerance of tobacco plants (Nicotiana plumbaginifolia) was investigated under in vitro culture conditions.
Material and Methods: Sterile seeds of N. plumbaginifolia were cultured in MS medium. In vitro propagated seedlings under tissue culture conditions were treated with IAA (mg / l) and salinity (0, 100 and 200 mM) for 4 weeks. Physiological and biochemical parameters including H2O2, chlorophyll, soluble carbohydrate, protein and antioxidant enzyme activity were measured.
Results: Salt stress increased H2O2, proline, catalase, superoxide dismutase and ascorbate peroxidase, while reverse patterns were observed for photosynthetic pigments, soluble carbohydrates and also total protein. In salt-treated seedlings, application of auxin increased proline, catalase and H2O2, but decreased photosynthetic pigmentation, soluble carbohydrates, total protein and some enzymes such as superoxide dismutase and ascorbate peroxidase.
Conclusion: Auxin treatment in salt-treated tobacco seedlings improved salinity tolerance by changes in some physiological parameters.