Cell and Tissue Journal

Abstract Aim: The aim of this study was to determine the effect of Carob seedextract as natural antioxidant on quality cryopreserved Farahani rams breeding sperm.
Material and Methods: In this study semen was collected from five mature ram (weight: 60±5 kg) twice a week using an artificial vagina and the ejaculates were pooled in order to eliminate the individual effect of rams. Different levels of carob seed extracts (0, 0/05, 0/1, 0/15 and 0/2 mL) were added to diluent based tris-egg yolk. After cooling, filling and sealing of the samples, they were frozen and with nitrogen vapor and immersed in liquid nitrogen and were stored until evaluation time. Thereafter and after thawing and incubation for5 min, sperm quality parameters includingmotility, progressive motility, viability (Nigrosine–eosin staining), membrane integrity with Hypoosmotic (Host) and morphology abnormality (Hancock test).
Results: Results indicated that the level of 0/05 mL carob seedextract significantly improved some parameters including motility, viability, plasma membrane and morphology integrity compared to control (p < 0.01).
Conclusion: Results indicated that so added 0/05 of carob seed extract peel to Tris based extender was beneficial in storage in sperm Farahani ram breeding after freeze-thawing.
 

Abstract Aim: The purpose of this study was to consider the effect of mild oxidative stress period on the frozen-thawed bull semen performance.
Material and Methods: In this study, 120 minutes was considered for equilibration of sperm before freezing. One µm of Nitric Oxide (NO) at the 0, 45, 90 and 120 cooling period was added to the diluant before cryopreservation. Sperm motion parameters, plasma membrane integrity, acrosome integrity, viability, apoptosis and mitochondria activity were assessed.
Results: Induction of oxidative stress with nitric oxide in the T0 and T45 groups led to significant improvement of total (88.4 ± 2.8, 84.7 ± 2.7) and progressive motility (50.4 ± 2.5, 49.5 ± 2.5) when compared to other groups. Percentage of plasma membrane integrity and viability were not different in T0 (74.4 ± 2.9 and 85.6 ± 2.9) and T45 (75.6 ± 2.9 and 84.6 ± 2.3) groups, where as it was significantly higher when compared with T90 (63.6 ± 2.9 and 69.6 ± 2.3) and T120 (61.5 ± 2.9 and 54 ± 2.3). Moreover, the highest significant percentage of spermatozoa with active mitochondria was observed in the T0 (82.5 ± 3.1) when compared with T45 (65.7 ± 3.1), T90 (42 ± 3.1) and T120 (43 ± 3.1). Acrosome integrity and linearity of sperm were not affected by the oxidative stress treatment time.
Conclusion: It seems that applying oxidative stress using 1 µM NO would improve post-thawed bull sperm quality at the T0 and T45 time of cooling.