Localization and Effect of Hyper and Hypo-osmotic Environments on Distribution of Na+, K+- ATPase in Kidney Tubules of Juveniles of (Epinephelus coioides)
Volume 2, Issue 4, Winter 2012, Pages 407-414
https://doi.org/10.52547/JCT.2.4.407
Abstract Aim: Histological study of kidney nephrons of Epinephelus coioides Juveniles and Immunolocalization of Na+, K+- ATPase during osmoregulation and adaptation with hypo- and hyper-osmotic (10 and 60 ppt) conditions.
Material and Methods: H & E staining and IgG 5 and FITC as primary and secondary antibodies were used for kidney nephrons histological studies and Immunolocalization of Na+, K+- ATPase, respectively.
Results: Results of microscopic study indicated that kidney nephrons of juvenile grouper were consisted of Glomerulus, Neck segment, Proximal, Distal and Collecting tubules. Immunolocalization of control group showed that Na+, K+- ATPase was distributed in all part of nephron structure but glomeruli. In hypo- and hyper-osmotic environments similar to control condition the enzyme distributed into the epithelial cells of proximal, distal and convoluted tubules but was not presence in glomeruli.
Conclusion: The results of present study indicated that, the structure of kidney nephrons of E. coioides was similarto that of other euryhaline species and presence of Na+, K+- ATPase in basolaterl portion of cell membrane indicated its active role in osmotic and ionic regulation in kidney.