Effect of iron oxid nanoparticle on the growth and physiology of inoculated alfalfa (Medicago sativa L.) with Rhizobium meliloti
Volume 11, Issue 1, Summer 2020, Pages 25-43
https://doi.org/10.52547/JCT.11.1.25
M Askary, SM Talebi, M Shafieigavari
Abstract Aim: The aim of this study is to evaluate the interaction of bacterial inoculation and iron treatment (nano and Fe-chelate) on physiological traits of alfalfa.
Material and Methods: In this study, effects of inoculation with standard Rhizobium meliloti, effects of different levels of iron (Fe-chelate, 0, 5, 10, 20 and 25 μM Fe2O3 nanoparticles) and the interaction of bacterial inoculation and iron treatment were investigated on alfalfa in a factorial experiment in completely randomized design with three replications for 45 days. The measured traits were growth indexes, photosynthetic pigments, protein, proline, antioxidants activity, DPPH(diphenyl-picryl-hydrazyl)-radical scavenging activity percent and elements content.
Results: Rhizobium incoculation alone showed beneficial effects on the alfalfa growth and was caused increasing in growth parameters, pigmants, protein content, potassium and phosphours uptake. However inoculation did not effect on the proline and antioxidant content. Iron treatment had a positive effect on the growth parameters, pigmants, protein content and elemant uptake. Highest values of growth parameters was observed 25μM Fe2O3 nanoparticles. The highest values of proline and antioxidants activity were measured in control (0μM nanoparticles). Since this concentration is considered a stress for alfalfa. Negative effects of 0μM nanoparticles decreased in inoculated alfalfa plants with R. meliloti. Indeed rhizobium causes increasing in inoculated plant resistant by reducing stressful conditions.
Conclusion: Rhizobium-alfalfa symbiosis plus iron nanofertilizer can cause increasing in plant resistance to stress, in addition to increase growth of plant. The highest amount of growth parameters, pigmants and protein content was measured in inoculated plant with Rhizobium meliloti and 10μM nanoparticles.
Study on toxic effects of diesel exhaust particles, on some growth factors and cellular responses (protein production) in the bean plant seeds and pollen
Volume 2, Issue 1, Autumn 2011, Pages 57-66
https://doi.org/10.52547/JCT.2.1.57
Abstract Aim: Air pollution is a global problem that has negative effects on living organisms. The aim of this study was to investigate the effect of water soluble fraction of diesel exhaust particles (DEP) on some growth factors and scale of bean’s protein.
Material and methods: Bean plants were cultivated in experimental condition and sprayed with 30 ml of different concentrations of DEP (0.1, 0.3, 0.6 g/L) and control plants were sprayed with distilled water. Growth factors such as wet and dry weights, length of legume, legume dry weight, seed germination and leaf area in DEP-treated and the control groups were compared with SPSS software and variance analysis. Pollen and seed proteins were studied with electrophoresis method.
Results: DEP treatments caused to decrease wet weights of the all DEP-treated plants (P≤0.05), but the other factors (pod length, dry pod weight, pod weight, seed germination and leaf surface) only in the treated group with a 0.6 g/L DEP was significantly reduced. Seed protein profiles were similar in the experimental and control samples, but an increase in the density of a band with molecular mass of 62 kDa was observed in the group treated with 0.6 g/L of DEP. In protein profile of pollen, a band with molecular mass of 55 kDa was disappeared and a new band with molecular mass of 80 kDa was formed.
Conclusion: With the consideration of reported detrimental effects for DEP, it seems that the bean plants are resistant that can be related to changes in protein banding profiles and cellular mechanisms of DEP detoxification.