Cell and Tissue Journal

Abstract Aims: In this study, the effects of Vitamin D3 on total protein concentration (TPC) and MBP expression in the corpus callosum extracts of Cuprizone induced demyelinated mouse has been investigated.
 Material and Methods: The mice were treated by Cuprizone for five weeks in order to induce demyelination. Then, the mice were divided into 3 groups. The first group was injected intraperitoneally (IP) by vitamin D3 in the amount of 5 µg/kg/daily body weight. The second group (SHAM) was injected IP by phosphate buffered saline (PBS) and the third group was left without injection as controls. After five weeks, the mice were killed and the corpus callosum was collected and the total protein concentration (TPC) and expression of MBP were studied by Western blot technique.
Results: No significant variation in the cortical TPC was seen in vitamin D3 injected group as compared to either SHAM or controls. We have also shown that the expression of MBP in the corpus callosum extracts of demyelinated mouse was significantly increased in the vitamin D3 injected group as compared to the other groups.
Conclusions: It is concluded that vitamin D3 increases MBP expression in the corpus callosum of cuprizone-induced demyelination mice. It is also suggested that vitamin D3 may play a role in the process of remyelination by increasing MBP expression in the cortex.
 
 

Abstract Aim: Vitamin D has an important role in treatment and prevention of metabolic syndrome which may enhance the benefits of sportive training. The purpose of this study was to determine the effects of 8 weeks of aerobic training with and without vitamin D supplementation on metabolic syndrome parameters in sedentary women.
Material and Methods:In this semi-experimental study with pretest–post designs, thirty obese middle-age women (aged 44.25±5.4 y) were randomly assigned to aerobic training only (RG), vitamin D supplementation (50000IU/w) plus aerobic training (VR) and control (non-exercising=CG) groups. Aerobic training program was performed 50-60 min/d, 3d/wk, for 2 months. Serum levels of 25-hydroxyvitamin D, body composition and metabolic parameters were assessed before and after the training period.
Results: Vitamin D supplementation increased serum 25-hydroxyvitamin D levels significantly (p < /em><0.05). Aerobic training caused significant improvement in body weight, waist circumference, HDL-cholesterol, blood glucose and insulin resistance index (p < /em><0.05). Vitamin D supplementation in conjunction with aerobic training lead to greater improvement in adiposity indices, HDL-cholesterol, LDL-cholesterol, total cholesterol, triglyceride, blood glucose and insulin resistance index (p < /em><0.05).
Conclusion: These results suggest that aerobic training is a safe and effective for improving cardiometabolic health in middle-age women. Also vitamin D combination can enhance some of the beneficial effects of training over a two-month period.