Cell and Tissue Journal

Abstract Aim: The aim of this study was to investigate the effect of secretory factors of human mesenchymal stem cells on gentamicin-induced acute kidney injury in rats.                                                                  
Material and Methods: Firstly, an experiment was designed to achieve a standard animal model. Animals were randomly divided into four groups. One group was considered as control which received no injection. Three other groups were treated with intraperitoneal 100 mg/kg gentamicin for six consecutive days. Then one of the three groups was injucted with conditioned media of human mesenchymal stem cells and other group was injucted with medium without secretory factors intraperitoneally for three consecutive days. Finally, in the third, fifth and eighth days after gentamicin injection, kidney and blood samples from all animals were collected for histological and biochemical analysis.
Result: Results, except in the special case, showed no significant decrease in the amount of blood biomarkers of animals in the group receiving the condition media of human mesenchymal stem cells compared to two another groups. The improvement of tissue damage, however, has been observed in the number of days.                                                                               
Conclusions: These findings suggest that secretory factors of human mesenchymal stem cells have aprotective effect against gentamicin-induced nephrotoxicity
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Abstract Aim: Cisplatin is one of the most successful drugs to fight many cancers, but its nephrotoxicity limits its administration. The aim of this study is investigation of the effect of human mesenchymal stem cells secretory factors on Cisplatin-induced acute renal failure in rats.
Material and Methods: In this study, male wistar rats were used. Animals were randomly divided into four groups and six animals were in each group. One group was considered as control which this group received no injections. Other three groups were treated with intraperitoneal 5 mg/kg Cisplatin once at the beginning of the experiment. Then one of these three groups was injected with conditioned medium of human mesenchymal stem cells and other groups were injected with medium without secretory factors intraperitoneally for three consecutive days. After five days Cisplatin injection, kidney and blood samples from all of examined animals were collected for histological and biochemical studies.
Result: Results showed significant decrease in the kidney injury level, blood serum BUN and creatinine amount. Also
Also body weight increasing significantly was observed in conditioned medium of human mesenchymal stem cells receiver group in comparison with treated Cisplatin and non secretory factors receiver groups.
Conclusion: Results of this research suggest that human mesenchymal stem cells secretory factors can be affect against Cisplatin-induced nephrotoxicity.