Cell and Tissue Journal

Abstract Aim: The aim of the present study was determination of FecB and FecG^H gene mutations in Kamouri goats of Khouzestan Province. Material and Methods: For this study 40 blood samples were collected of prolific kamouri goats. DNA of blood samples was extracted by modified salting out method and the site of the FecB and GDF9 genes were amplified using specific primers. Results: After amplification of the site of genes, the products of 190 bp and 139 bp were determined by agarose gel electrophoresis. The PCR products were digested with AvaII and DdeI enzymes. Results show no mutations in the FecB and FecG^H genes in the Khouzestan kamouri goats. Conclusion: In this study the mutations of FecB and FecG^H are not observed in Khouzestan kamouri goats and all of the goats were wild type. Therefore these mutations are not the general cause of the prolificacy in the Khouzestan Kamouri goats and further investigations are required to evaluating the fecundity genes and the genotyping of Khouzestan Kamuori goats.