Effect of Embryonic Cerebrospinal Fluid on Proliferation and Self-Renewal of Wistar Rat Neuroprogenitor Cells
Volume 4, Issue 3, Winter 2013, Pages 305-311
https://doi.org/10.52547/JCT.4.3.305
Abstract Aim: During recent years many studies have suggested some developmental roles for embryonic cerebrospinal fluid. Here we examined the effect of embryonic cerebrospinal fluid on proliferation and self-renewal of embryonic ventricular zone derived neurosphere.
Material and Methods: Cortex from 15.5 day old embryonic Wistar rat dissected out and enzymaticaly dissociated to form single cell suspension. Cell suspension seeded in DMEM/F12 medium supplemented with N2 and mitogenes (10ng.ml-1 EGF and 20ng.ml-1 bFGF). The CSF-treated culture from different embryonic ages (E16, E18, and E20) in 10/100 ratio (v/v) was considered as experimental groups. Neurospheres number was counted for proliferation assay and cell viability evaluated with MTT assay. The data were analyzed with One-way ANOVA with turkey’s post hoc test.
Results: Embryonic cerebrospinal fluid (e-CSF) enhanced neurosphere number. Also, embryonic cerebrospinal fluid (e-CSF) enhanced cell viability and growth.
Conclusion: Embryonic cerebrospinal fluid (e-CSF) enhanced proliferation and viability of neural progenitor cells in age dependent manner. Moreover, this fluid play important role in establishment of neuroprogenitor cells self-renewal.