Bioinformatics and Molecular Cytogenetic Study of a Small Region of Chromosome 15 Including Allelic Imbalances in Uterine Cancer Prone Inice
Volume 4, Issue 3, Winter 2013, Pages 297-303
https://doi.org/10.52547/JCT.4.3.297
Abstract Aim: Previous studies using LOH technique on BDII rats showed four distinct Allelic imbalance on chromosome 15. In this research we have studied region 2 including Allelic imbalance by bioinformatic approachs and FISH (Florescence in situ hybridization) technique.
Material and Methods: The confirmed tumors by an expert pathologist were used for cell culture. Metaphase chromosomes were prepared by conventional methods. The corresponding genes labeled probe for hybridization was poured onto slides. The labeled probe was then subjected to detected phase. Slides were studied under a fluorescence microscope using software CW4000 Laica.
Results: Using some of the database we could register 104 genes. But most of them did not have a clear function. According of FISH results, Dlgap5, Fermt2 and Socs4 had amplification and Lgals3 had copy number reduction.
Conclusion: according to our results, the Dlgap5 and Socs4 are probably involve in EAC